大剂量岷当归对高同型半胱氨酸血症致兔动脉粥样硬化的影响

The Effects of MinDangGui in Large Doses on the Rabbits with HHcy-induced AS

目的:探讨大剂量岷当归对高同型半胱氨酸血症(HHcy)致兔动脉粥样硬化(AS)的影响。方法:将日本大耳白兔48只随机分为正常组、模型组、叶酸组及岷当归低、中、高剂量组,每组8只。采用L-蛋氨酸皮下注射法建立HHcy家兔模型。造模8周后,岷当归低、中、高剂量组分别给予1.4、2.8、5.6 g/(kg·d)的岷当归水煎液灌胃治疗,叶酸组给予叶酸1.5 mg/(kg·d)、维生素B620 mg/(kg·d)、维生素B120.25 mg/(kg·d)灌胃治疗。12周后检测各组血清同型半胱氨酸(Hcy)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)水平,同时观察兔腹主动脉血管超声与病理变化。结果:模型组兔血清Hcy水平高于正常组,差异有统计学意义(P<0.05),HHcy模型形成;岷当归低、中、高剂量组及叶酸组血清Hcy水平与模型组比较下降,差异有统计学意义(P<0.05);岷当归低、中、高剂量组血清TC、TG、LDL-C水平低于模型组,差异有统计学意义(P<0.05);腹主动脉超声与组织学检查发现,模型组有明显的AS征象,各治疗组病变程度较模型组减轻。结论:大剂量岷当归通过降低血清中Hcy水平,调节脂肪代谢紊乱,减轻HHcy对血管内皮的损伤,对HHcy所致的AS病变起到治疗作用。

Objective： To explore the effects of MinDangGui （angelica sinensis） in large doses on the rabbits with hyperhomocystinemia （HHcy）-induced atherosclerosis （AS）. Methods： All 48 rabbits were randomly allocated to the normal group, the model group, the control group of folacin, low, moderate and high dosages groups of Min- DangC, ui, eight rabbits each group. HHcy rabbit model was prepared by using hypodermic injection of L-methionine. After successfully preparing the rabbit model in eight weeks, low, moderate and high dosages groups of MinDangGui accepted intragastric administration of the decoction of MinDangGui in the concentrations of 1.4, 2.8 and 5.6 g/（kg-d） respectively, and the control group of folacin intragastric administration of folacin, 1.5 g/（kg od）, vitamin B6, 20 g/（kg.d） and vitamin B12, 0.25 g/（kg＇d）. The levels of Hcy, TC, TG, HDL-C and LDL-C of different groups were detected in 12 weeks, simultaneously, pathological changes and abdominal aorta vascular ultrasound of the rabbits were observed. Results： The model group was higher than the normal group in the level of Hey, and the difference had staisitical meaning （P 〈0.05）, and HHcy model has been formed; the levels of Hey decreased when low, moderate and high dosages groups of MinDangCui, the control group of folaein were compared with the model group, and the difference showed staisitical meaning （P 〈0.05）; low, moderate and high dosages groups of MinDangGui were lower than the model group in the levels ofTC, TG and LDL-C, and the difference showed staisitical meaning （P〈0.05）; abdominal aorta vascular ultrasound and histological examination indicated that there was notable AS signs in the model group, pathological changes of different treatment groups were lighter than these of the model group. Conclusion： MinDangGui in large doses could treat HHcy-induced AS by decreasing the levels of Hey, regulating fat metabolism disorder and relieving the damage to blood vessel endothelium by HHcy.