去氢木香内酯对人肝星状细胞增殖及凋亡的影响研究

Effect of dehydrocostuslactone on proliferation and apoptosis in hepatic stellate cells

目的探讨去氢木香内酯(Dehy)对人肝星状LX-2细胞增殖及凋亡的影响,分析其可能的作用机制。方法人肝星状LX-2细胞经不同浓度Dehy处理后,采用四甲基偶氮唑盐(MTT)法、流式细胞术检测LX-2细胞增殖和周期分布,Hoechst33342染色法及AV-PI双染法检测LX-2细胞凋亡情况,蛋白质印迹法(Western blot)检测凋亡相关蛋白的表达。结果给予不同浓度的Dehy作用48h后,能够明显抑制LX-2细胞的增殖,阻滞细胞周期于S、G2/M期,同时诱导LX-2细胞的凋亡,呈浓度依赖性;Western blot结果显示,Dehy可促进P27、Bax的表达上调,Bcl-2的表达下调。结论 Dehy通过调节Bax、Bcl-2和P27的表达诱导人肝星状LX-2细胞的凋亡和周期的阻滞。

Objective To investigate the effects of dehydrocostuslactone（Dehy） on human hepatic stellate LX-2 cells prolif- eration,apoptosis and the possible related mechanisms. Methods After treating the LX-2 cells by different concentrations of Dehy, the MTT assay and flow cytometry were used to assess the influence of Dehy on cell proliferation and cycle distribution of LX-2 cells. The Hoechst 33342 staining and AV-PI dual staining were used to detect the effect of Dehy on ceil apoptosis in LX-2 cells. The apoptosis related proteins expression was detected by Western blot. Results After different concentrations of Dehy acting for 48 h could significantly inhibit the proliferation of LX-2 cells,blocked the cellular cycle at stage S and G2/M, meanwhile induced LX-2 cells apoptosis in the concentration dependent manner; Western blot results showed that Dehy could promote the up-regulation of P27 and hax expression and down-regulation of Bcl-2. Conclusion Dehy induces human liver LX-2 cells apoptosis and cell cycle arrest by adjusting the expression of Bax,Bcl-2 and P27.