禽类转录因子与DNA互作的研究方法

Methods of Transcription Factors and DNA Interactions in Poultry

在禽类生长发育中,基因的适时适量表达起到至关重要的作用。研究转录因子与DNA的互作,对认识禽类生长发育过程具有重要意义。文章总结了禽类常用的转录因子与DNA互作研究方法,其中:生物信息学预测结合位点有助于针对性的开展后续研究;电泳迁移率变动分析(Electrophoretic mobility shift assay,EMSA)和染色质免疫共沉淀(Chromatin Immunoprecipitation,ChIP)能够分别从体外和体内验证转录因子与DNA结合;双荧光素酶报告基因可以鉴定对转录活性的影响。综合运用上述方法可有效开展转录因子-DNA互作研究,有助于揭示禽类生长发育调控机制。

Timely and proper gene expressions play a great part in poultry growth and development. Study the binding of transcription factors and DNA is contributed to recognize the poultry growth process. Our article summarized the current commonly methods of transcription factors and DNA interactions. Bioinformatics prediction of transcription factor binding sites help target to the follow-up experiments. Electrophoretic mobility shift assay （EMSA） and chromatin immunoprecipitation （CHIP）can respectively detect the binding of transcription factors and DNA in vitro and in vivo. Dual luciferase reporter gene assay could reveal the transcription activities of the binding sites. Together, combining the above methods could carry out the transcription factors and DNA interaction analysis effectively, and help to re- veal the mechanism of poultry development and growth step by step.