摘要
以24个羽衣甘蓝品种为试验材料,测定低温胁迫后叶片相对电导率、可溶性糖含量、可溶性蛋白质含量、脯氨酸含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性等生理生化指标,以各项指标的耐寒系数衡量羽衣甘蓝品种的耐寒性,并利用主成分分析和隶属函数分析法对其耐寒性进行综合评价。结果表明:经过低温胁迫后,叶片相对电导率、可溶性糖含量、可溶性蛋白质含量、脯氨酸含量、MDA含量、SOD活性均高于对照,且各品种间差异显著。参试品种的综合分值(D值)介于0.15到0.60之间。根据D值将24个品种分为4类:Y108、Y4389、霓裳和Y93,耐寒性极强;名古屋红、瑞羽6号、Y92、Y122、Y4382、Y90和Y114,耐寒性强;Y4330、Y4496、Y130、Y4467和Y105,耐寒性中等;红欧、瑞羽11号、白鸥、白鸽、Y9197、Y9196、Y112和名古屋白,耐寒性弱。相关性分析结果显示,D值与植株叶片可溶性糖含量、脯氨酸含量及SOD活性呈极显著正相关(P<0.01),与可溶性蛋白质含量呈显著正相关(P<0.05)。因此,植株叶片的可溶性糖含量、脯氨酸含量、SOD活性、可溶性蛋白质含量可以用于综合评价羽衣甘蓝的耐寒性。
Using 24 kale varieties as material,the effect of low temperature stress on cabbage leaf relative electrical conductivity,soluble protein content,soluble sugar content,proline content,malondialdehyde(MDA) content and super oxide dismutase(SOD) activity was determinated. The cold tolerance of kale was measured by cold resistant coefficient of each index and comprehensively evaluated by using principal component analysis and membership function analysis. The results showed that leaf relative electrical conductivity,soluble protein content,soluble sugar content,proline content,MDA content and SOD activity of kale in the group of low temperature stress were higher than those in the group of control,and there was significant difference among the different varieties. Comprehensive score(D value) was between 0. 15 to 0. 60.According to the D value,24 kale materials could be divided into four types,including Y108,Y4389,Nishang and Y93 as strong cold resistance,Mingguwu red,Ruiyu6,Y92,Nagoya Y122,Y4382,Y90 and Y114 as cold resistance,Y4330,Y4496,Y130,Y4467 and Y105 as the medium cold resistance,Hongou,Ruiyu11,Baiou,Baige,Y9197,Y9196,Y112 and Mingguwu white as the low cold resistance. Correlation analysis showed that the D value had extremely significant positive correlation(P<0. 01) with soluble sugar content,proline content and SOD activity,and had significantly positive correlation(P< 0. 05) with the soluble protein content. In conclusion,soluble sugar content,proline content,SOD activity soluble protein content can be used as comprehensive evaluation index of kale cold tolerance.
出处
《江苏农业学报》
CSCD
北大核心
2017年第6期1349-1357,共9页
Jiangsu Journal of Agricultural Sciences
基金
镇江市国际合作项目(GJ2015009)
关键词
羽衣甘蓝
耐寒性
生理响应
综合评价
kale
cold tolerance
physiological response
comprehensive evaluation