摘要
为了解华南虎源大肠杆菌的耐药性与致病性情况,采集龙岩梅花山虎园老虎粪便分离大肠杆菌,并对分离株进行药敏试验、动物毒力试验以及毒力基因检测。结果表明,分离株对复方新诺明(100%)、多西环素(75%)和阿莫西林/克拉维酸(75%)产生耐药;对庆大霉素的敏感性较好;对头孢呋辛和头孢噻呋敏感性下降。动物毒力试验显示,分离株具有较强的致病性,同时毒力基因aat A的检出率为100%,irp2检出率为75%;papC、iucD、vat和iss检出率均为50%,tsh和cva/cvi未检出。虎源大肠杆菌对常用药物具有一定的耐药性,同时表现出较强的致病性,其毒力基因的携带率较高。该结果为兽医临床合理用药提供一定的科学依据。
The aim of the study was to study the antimicrobial resistance and pathogenicity among Escherichia coli isolated from Southern China tiger＇ s feces in Meihua mountain nature reserve, Longyan. Those isolates were tested for antimicrobial susceptibility and animal toxicity, and screened for virulence genes. Results showed that isolates had resistance to Trimethoprim-sulfamethoxazole （100%）, doxycycline （75%） and amoxicillin/clavulanic acid （75%）. However those isolates were susceptible to gentamicin and had reduced susceptibility to cefuroxime and ceftiofur. The animal toxicity test showed that these strains have strong pathogenicity, and virulence genes, such as aatA（lO0% ）, irp2（75% ）, papC（50% ）, iucD（50% ）, vat（50% ） and iss（50% ） were also found. Howerer, tsh and cva/cvi were not detected. Escherichia coli isolated from Southern China tiger had a certain degree of antimicrobial resistance, strong pathogenicity and a high carrying rate of virulence genes. Those results provided the important dates and theoretical basis for veterinary medicine.
出处
《中国兽医杂志》
CAS
北大核心
2017年第11期73-75,I0004,共4页
Chinese Journal of Veterinary Medicine
基金
福建省科技厅青年基金项目(2010J05077)
龙岩市科技局青年人才项目(2013LY45)
关键词
药物敏感性试验
抗菌药
动物毒力试验
毒力基因
耐药性
antimicrobial susceptibility
antibiotics
animal toxicity test
virulence genes
resistance
