摘要
目的研究索拉非尼对不同人肝癌细胞株Hep G2、Hep3B、BEL-7402、BEL-7404、BEL-7405、QGY-7701、QGY-7703、SMMC-7721、MHCC97H、MHCC97L、HCCLM3、HCCLM6中B7-H3和B7-H4蛋白表达的影响。方法免疫印迹法和溴化噻唑蓝四氮唑(MTT)比色法检测索拉非尼在不同人肝癌细胞株中对B7-H3和B7-H4蛋白表达的影响及索拉非尼对不同人肝癌细胞株的抑制作用。结果不同人肝癌细胞株中B7-H3和B7-H4蛋白表达较正常人肝细胞(HL-7702)均显著上调(P<0.01)。索拉非尼对Hep3B、BEL-7404、MHCC97H、HCCLM3和HCCLM6肝癌细胞株的细胞毒活性IC_(50)值分别为14.56、9.14、9.46、17.21、9.29μmol/L。分别以5、10、20μmol/L剂量索拉非尼处理Hep3B、BEL-7404、MHCC97H、HCCLM3、HCCLM6后,B7-H3和B7-H4蛋白表达均较对照组显著下调(P<0.01)。结论 B7-H3和B7-H4蛋白在不同人肝癌细胞株中通常过表达,可影响肿瘤免疫逃逸,可能是未来肝癌防治的一个新靶点。
Objective To investigate the influence of sorafenib on the expressions of B7-H3 and B7- H4 proteins in different human hepatocellular carcinoma cell lines, including HepG2, Hep3B, BEL-7402, BEL-7404, BEL-7405, QGY-7701, QGY-7703, SMMC-7721, MHCC97H, MHCC97L, HCCLM3 and HCCLM6. Methods Western blotting and MTT assay were used to check the influence of sorafenib on the expressions of B7-H3 and B7-H4 proteins in different human hepatocellular carcinoma cell lines and to test the inhibitory effect of sorafenib on different human hepatocellular carcinoma cell lines. Results Compared with normal human liver cells （HL-7702）, the expressions of B7-H3 and B-H4 proteins in different human hepatocellular carcinoma cell lines were significantly up-regulated （P〈O.01）. The cytotoxic activity ICso values of sorafenib to Hep3B, BEL-7404, MHCC97H, HCCLM3 and HCCLM6 were 14.56, 9.14, 9.46, 17.21 and 9.29 p^mol/L respectively. After treating Hep3B, BEL-7404, MHCC97H, HCCLM3 and HCCLM6 with sorafenib at the doses of 5, 10 and 20 μmol/L separately, the expressions of BT-H3 and B7-H4 proteins were strikingly down-regulated when compared with the control group （P〈0.01）. Conclusion The over- expressions of B7-H3 and B7-H4 proteins in different human hepatocellular carcinoma cell lines are a common finding, which can influence tumor immune escape. It may be a new target for prevention and treatment of liver cancer in future.（J Intervent Radiol, 2018, 27： 58-62）
出处
《介入放射学杂志》
CSCD
北大核心
2018年第1期58-62,共5页
Journal of Interventional Radiology
基金
贵州省普通高等学校工程研究中心项目(黔教合KY2016-012)
