HPLC法同时测定六经头痛片中4种成分

Simultaneous determination of four constituents in Liujing Toutong Tablets by HPLC

目的建立HPLC法同时测定六经头痛片(白芷、辛夷、藁本等)中4种成分的含有量。方法该药物30%乙醇提取液的分析采用Waters C18色谱柱(4.6 mm×250 mm,5μm);以甲醇-4%乙酸为流动相,梯度洗脱;检测波长320 nm;体积流量1.0 m L/min;柱温35℃。结果葛根素、阿魏酸、欧前胡素、异欧前胡素分别在60.6~303μg/m L(r=0.999 9)、1.59~7.95μg/m L(r=0.999 9)、1.57~7.85μg/m L(r=0.999 9)、0.752 5~3.762 5μg/m L(r=0.999 7)范围内呈良好的线性关系,平均加样回收率(RSD)分别为97.75%(1.7%)、97.68%(2.3%)、97.94%(1.0%)、98.29%(1.6%)。结论该方法稳定可靠,可用于六经头痛片的质量控制。

AIM To establish an HPLC method for the simultaneous content determination of four constituents in Liujing Toutong Tablets（ Angelicae dahuricae Radix,Magnoliae Flos,Ligustici Rhizoma et Radix,etc.）.METHODS The analysis of 30% ethanol extract of this drug was performed on a 35 ℃ thermostatic Waters C18 column（ 4. 6 mm × 250 mm,5 μm）,with the mobile phase comprising of methanol-4% acetic acid flowing at1. 0 m L/min in a gradient elution manner,and the detection wavelength was set at 320 nm. RESULTS Puerarin,ferulic acid,imperatorin and isoimperatorin showed good linear relationships within the ranges of 60. 6-303 μg/m L（ r = 0. 999 9）,1. 59-7. 95 μg/m L（ r = 0. 999 9）,1. 57-7. 85 μg/m L（ r = 0. 999 9） and0. 752 5-3. 762 5 μg/m L（ r = 0. 999 7）,whose average recoveries（ RSDs） were 97. 75%（ 1. 7%）,97. 68%（ 2. 3%）,97. 94%（ 1. 0%） and 98. 29%（ 1. 6%）,respectively. CONCLUSION This stable and reliable method can be used for the quality control of Liujing Toutong Tablets.