miR-17-92启动子区多态性在广西人群中分布及与淋巴细胞关系

Distribution of polymorphisms at promoter region of miR-17-92 in Guangxi population and association of its polymorphisms and lymphocytes

目的:研究广西人群中miR-17-92基因簇启动子区rs9515692C/T和rs1352743A/G多态性分布特点和在不同人群间分布差异,探讨其多态性和淋巴细胞的关系。方法:采取多重单碱基延伸法(SNaPshot)和DNA测序法进行基因分型。运用流式细胞仪检测淋巴细胞数。数据分析利用SPSS17.0统计学软件。结果:2位点基因型及等位基因频率在广西人群的不同性别间差异无统计学意义(P>0.05)。rs9515692C/T和rs1352743A/G基因型及等位基因频率与国际人类基因组单体型图计划(HapMap)公布的欧洲、日本和非洲人群的比较差异有统计学意义(P<0.05)。结论:rs9515692C/T和rs1352743A/G基因多态性在不同人群中存在着不同程度差异。此外,rs9515692C/T多态性与B淋巴细胞数有关。

Objective： To investigate the distribution characteristics of polymorphisms of rs9515692 C/T and rs1352743 A/G in the promoter region of miR-17-92 gene cluster in Guangxi people and compare them with those of other ethnic groups and explore the association of its polymorphisms and lymphocytes. Methods： The rs9515692 C/T and rs1352743 A/G of miR-17-92 gene cluster were genotyped by using SNa Pshot technique and DNA sequencing. Detection of the number of lymphocytes using flow cytometry. The differences of polymorphisms between groups were analyzed statistically. Results： No significant differences of genotype and allele frequency in the two SNPs was observed between different gender in the Guangxi population（ P 〉0. 05）. However,there were significant differences in the distribution frequencies of genotype and allele of Europeans,Japanese and Africans in rs9515692 C/T and rs1352743 A/G（ P〈 0. 05）. Conclusion： Polymorphisms of rs9515692 C/T and rs1352743 A/G are different in different people. In addition,rs9515692 C/T polymorphism may be associated with the number of B cells.