武汉地区丙型肝炎病毒包膜蛋白E1的生物信息学研究

Bioinformatic study of hepatitis C virus envelope protein E1 in Wuhan

使用生物信息学的方法,分析武汉地区不同基因型、亚型的丙肝病毒的包膜E1蛋白,预测其二级结构、核苷酸变异性、糖基化位点、亲水性、跨膜区、信号肽、蛋白修饰位点、B细胞抗原.结果显示各HCV的包膜E1蛋白二级结构差距不大;序列始末段有数个氨基酸残基的差距,序列上存在高变异位点,基因型2a的型内一致性最高;序列大量糖基化,有多个糖基化基化位点;序列分布着亲水性区域,基因型1b的分值很高;基因型1b、6a、3b、3a多数亚型有1个跨膜区,基因型2a多数亚型有两个跨膜区;基因型1b、6a、3b、3a无信号肽,基因型2a都有信号肽;蛋白序列上存在多个不同修饰位点和B细胞抗原,各基因型、亚型之间有明显差距,具有较大异质性.该研究为揭示病毒感染机制和研制地区性疫苗提供一定的科学依据.

The envelope proteins E1 of different HCV genotypes and subtypes in Wuhan were analyzed bioinformatically to predict their secondary structure,nucleotide variability,glycosylation site,hydrophilicity,transmembrane domain,signal peptide,protein modification site,and B cell antigen.The results indicated that envelope proteins E1 of HCVs possess little secondary structure difference;the beginning segment of the sequence is different from the end segment by several amino acid residues,with hypervariable site in the sequence;the genotype 2 ashows the highest consistency;the sequence shows significant glycosylation with multiple glycosylation sites;hydrophilicity areas distribute in the sequence and the genotype 1 bshows high score;most subtypes of genotypes 1 b,6 a,3 b,3 ahave one transmembrane domain,while 2 ahave two;genotypes1 b,6 a,3 b,3 ahave no signal peptide,while 2 ahas;multiple different modification sites and B cell antigens in the protein sequence,which varies with the genotypes and subtypes,exhibiting significant heterogeneity.The study has provided references for demonstrating mechanism of HCV infection and development of region-specific vaccines.