摘要
本试验旨在对陆川猪G蛋白偶联受体1(G protein-coupled receptor 1,GPR1)基因进行克隆及相关生物信息学分析。本研究根据NCBI上公布的野猪GPR1基因序列设计引物,应用RT-PCR技术扩增得到包含全长编码区在内的基因片段,应用生物信息学软件对陆川猪GPR1基因的理化性质,修饰结构,二级结构和三级结构进行分析。结果显示:GPR1基因编码区全长1 068 bp,编码355个氨基酸;与NCBI上公布的野猪(NM_001190244.1)、牛(NM_001206545.1)、人(NM_005279.3)、猕猴(AF100204.1)、小鼠(NM_146250.2)、大鼠(NM_012961.1)GPR1基因氨基酸序列的同源性分别为98.9%、90.4%、86.5%、86.2%、78.2%、77.4%。陆川猪的GPR1蛋白有七个跨膜螺旋结构,不存在蛋白信号肽。GPR1蛋白存在两个N糖基化位点和多个潜在磷酸化位点。本研究成功克隆陆川猪GPR1基因完整的编码区序列,为今后GPR1基因在陆川猪的脂肪沉积及脂肪代谢方面的研究提供了理论依据。
The objective of this study was to clone the G protein-coupled receptor 1(GPR1) gene of Luchuan pig and analyze its related bioinformatics.The GPR1 primers of this study were designed according to the wild boar GPR1 gene sequences published in NCBI.The GPR1 gene fragment including the full-length coding region was amplified by RT-PCR and the physicochemical property,modification structure,secondary structure and tertiary structure of GPR1 gene were analyzed by bioinformatics software.The results showed that GPR1 gene fragment included an 1068 bp whole length coding sequence and encoded 355 amino acids.The Luchuan pig CPR1 shared98.9%,90.4%,86.5%,86.2%,78.2% and 77.4% of the homology of amino acid sequence with that of Sus scrofa(NM_001190244.1),Bos taurus(NM_001206545.1),Homo sapiens(NM_005279.3),Macaca mulatta(AF100204.1),Mus musculus(NM_146250.2) and Rattus norvegicus(NM_012961.1),respectively.Luchuan pig GPR1 protein had seven transmembrane helical structures and no signal peptide.There were two N-glycosylation sites and several potential phosphorylation sites in GPR1 protein.This study suggested that the whole length coding sequence of Luchuan pig GPR1 gene was successfully cloned,and might provide theoretical references for further studying on the fatty deposition and lipid metabolism of GPR1 gene in Luchuan pig.
作者
谢婉
何剑雄
陈宝剑
关志惠
郭亚芬
兰干球
谢炳坤
Xie Wan;He Jianxiong;Chen Baojian;Guan Zhihui;Guo Yafen;Lan Ganqiu;Xie Bingkun(College of Animal Science and Technology,Guangxi University,Nanning,530004;Guangxi Key Laboratory of Livestock Genetic Improvement,Guangxi Institute of Animal Sciences,Nanning,530001)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2018年第8期3262-3268,共7页
Genomics and Applied Biology
基金
国家自然科学基金项目(31360544)资助