襄麦冬多糖及皂苷体外活性研究

Study on Activities of Polysaccharide and Liriopesides B from Liriope Spicata Lour. var. Prolifera Y. T. Ma in Vitro

用热水浸提醇沉法提取襄麦冬多糖并研究其体外抗氧化活性;利用滤纸片法研究不同浓度的襄麦冬皂苷B的抑菌活性。结果显示,襄麦冬多糖具有一定的体外抗氧化活性,其中对超氧阴离子自由基清除能力最强,高达70.83%,其次为对羟自由基的清除能力(25.69%),对DPPH自由基的清除能力最弱。浓度为2.0、4.0 mg/mL的襄麦冬皂苷B对大肠杆菌和金黄色葡萄球菌均具有抑菌作用,对大肠杆菌的抑菌圈直径分别为(10.83±0.82)mm,(14.89±0.50)mm;金黄色葡萄球菌的抑菌圈直径分别为(11.63±0.33)、(17.05±0.67)mm,在相同浓度下,金黄色葡萄球菌对麦冬皂苷的敏感性比大肠杆菌强。研究结果表明,襄麦冬多糖及皂苷B分别具有明显的抗氧化作用和抑菌活性。

Polysaccharide fromLiriope Spicata Lour. var. Prolifera Y. T. Ma was extracted via hot water extraction and alcohol precipitation method,and its antioxidant activities in vitro were studied. Bacteriostatic experiments of different concentrations liriopesides B was performed by the method of filter paper. Results showed that polysaccharide exhibited different antioxidant activity,with maximum scavenging ability on superoxide anion free radical,up to 70.83 %,medium scavenging capacity on hydroxyl radical（25.69 %）,and minimum on DPPH free radical. Different concentrations liriopesides B（2.0 and 4.0 mg/mL）showed obvious inhibitory effect on both bacteria,bacteriostatic ring diameter of Escherichia coli were（10.83±0.82）mm and（14.89 ±0.50）mm respectively,and the diameter of Staphylococcus aureus were（11.63 ±0.33）mm and（17.05 ±0.67） mm respectively. At the same concentration,Staphylococcus aureus was more sensitive to liriopesides B than Escherichia coli. In short,polysaccharide and liriopesides B fromLiriope Spicata Lour. var.Prolifera Y. T. Ma showed obvious antioxidant and bacteriostatic activities.