PCR技术检测泌尿生殖道生殖支原体(MG)感染的研究

On Mycoplasma Genitalium Infection of Urogenital Tract Detected by Technique of PCR

目的采用PCR检验技术,对吉林地区部分非淋菌性尿道(宫颈)炎(NGU)高危人群泌尿生殖道分泌物标本进行分离鉴定,以探讨MG感染在STD的作用和地位.方法吉林地区NGU高危人群受检者141例,宫颈或尿道口内拭子分泌物标本用PCR技术进行DNA扩增,并与对照组65例进行比较,结果141例标本MG-DNA扩增出26例,阳性率为18.4%,正常对照组65例MG-DNA扩增出3例,阳性率为4.62%(3/65),差异显著(x2=5.933,P=0.014 9＜0.05).结论1)吉林地区STD高危人群有较高的MG感染率,是NGU的病因之一2)PCR技术具有敏感、快速、特异性强特点,可以用来研究MG感染现状,流行情况及致病机制.

Objective Technique of PCR was used to study Mycoplasma genitalium(MG) infection of urogenital tract specimens taken from STD clinics and promiscuous persons in Jilin regions.Methods Specimens from 141 cases and 65 normal persons were detected for MG DNA.Results The MG DNA positive rate of 141 cases (26/141,18.4%) was much higher than that in control group (3/65,4.62%;x 2=5.933, P =0.014?9<0.05).Conclusion 1)There is a higher rate of MG infection in STD high risk population in Jilin regions and it is one of pathogenic reasons of NGU;2)The technique of PCR could be used to study the infectious and epidemic conditions about MG with its sensitive,quick and specific properties.