极低出生体质量新生儿血浆Uu-DNA拷贝数和IL-6检测的临床意义

Clinical Significance of Plasma Uu-DNA Copy Number and IL-6 Detection in Very Low Birth Weight Infants

目的探讨极低出生体质量新生儿(VLBWI)血浆解脲脲原体(Uu)-DNA拷贝数和IL-6检测的临床意义。方法选取80例胎龄≤32周的VLBWI,于生后1h内及第3、7、14、28天分别取静脉血标本,用FQ-PCR检测UuDNA拷贝数,用ELISA法测定感染相关的血浆IL-6水平,并分析Uu-DNA检测阳性与VLBWI的常见并发症支气管肺发育不良(BPD)、早产儿脑室内出血(IVH)及新生儿全身炎症反应综合征(ISIRS)的相关性。结果从出生后1h至28d新生儿Uu-DNA阳性率逐渐下降;Uu-DNA检测阳性组BPD、IVH、ISIRS的发生率及血浆IL-6水平均高于Uu-DNA检测阴性组(P<0.05)。结论血清中Uu水平可随新生儿出生后自行下降,Uu检测阳性与VLBWI的BPD、IVH和ISIRS、血浆中炎性细胞因子水平等关系密切。

Objective To investigate the clinical significance of plasma Uu-DNA copy number and IL-6 detection in very low birth weight infants (V L B W I). Methods Eighty VLBWI infants (gestational age^ 32 weeks) were selected in this study. Venous blood samples were taken within 1 hour and on days 3 ,7 ,1 4 and 28 days after birth. Uu-DNA copy number and plasma IL-6 level were detected by FQ-PCR and ELISA, respectively. In addition, the relationships of positive Uu-DNA to bronchopulmonary dysplasia(BPD) , intraventricular hemorrhage(IVH) and Infants systemic inflammatory response syndrome(ISIRS) were analyzed. Results The positive rate of Uu-DNA decreased over time postpartum. Compared with Uu-DNA negative group, the incidence of BPD ,IVH , ISIRS and plasma IL-6 level significantly increased in Uu-DNA positive group (_P〈0. 05). Conclusion Serum concentration of Uu decreases over time postpartum. The positive Uu-DNA is closely correlated with BPD ,IVH , ISIRS and plasma inflammatory cytokine levels in VLBWI infants.