谷甾醇对斑马鱼生长发育和生殖的影响

Effects of Sitosterol on the Growth and Reproduction of Barchydanio rerio var

为了研究谷甾醇(sitosterol)暴露对斑马鱼(Barchydanio rerio var)内分泌的干扰效应,通过不同浓度的谷甾醇(50、100、150、200μg/L)对雌性斑马鱼进行为期60 d的水浴暴露,同时设乙醇对照,分别于60 d后测定各组分雌性斑马鱼的生长指标,对部分组织进行切片观察,从组织病理学和毒理学方面评估谷甾醇暴露的毒性效应。结果表明:在200μg/L谷甾醇暴露下,斑马鱼全部死亡,其他各浓度处理与对照组相比,除臀鳍数随谷甾醇浓度的增加呈现倒"U"型趋势,以及肝重随谷甾醇浓度的增加而上升外,其他各生长指标均不同程度受到抑制,抑制作用与暴露浓度呈明显的剂量效应。此外,性腺指数(GSI)呈下降趋势,肝体指数(HSI)呈上升趋势,各组织切片也表现出明显的毒性反应。因此,可初步判断谷甾醇对斑马鱼的生长发育和繁殖系统会产生较大影响。

The effect of sitosterol on physiological indexes, HSI and GSI in the liver of Barchydanio rerio var were investigated. The female were selected and exposed to 50, 100, 150 μg/L and 200 μg/L sitosterol for 60 d by using the hydrostatic bath method. The results showed that except for all death of the Barchydanio rerio var in the solution under concentration of 200 μg/L, there was dose effect relationship between physiological indexes and sitosterol concentration within a certain concentration range. The paper also indicated that GSI presents a trend of increasing and the HSI presents opposite, and obvious toxic effects were presented in the tissue section figures. It is concluded that sitosterol effects the growth and reproductive system of Barchydanio rerio var.