微波增敏的mPEG-PLGA栓塞微球治疗原发性肝癌的研究

Microwave-sensitive mPEG-PLGA Embolic Microspheres for Hepatocellular Carcinoma Therapy

本文通过复乳法制备了具有微波增敏功能的单甲氧基醚聚乙二醇-聚乳酸羟基乙酸(mPEG-PLGA)栓塞微球,用于原发性肝癌的治疗研究。mPEG-PLGA微球生物安全性良好,呈较均匀的单分散性,平均粒径约为63μm。与对照组相比,mPEG-PLGA微球在微波辐射下能升高8.5℃。以ICR小鼠皮下H22肿瘤为模型,在微球辅助下,微波消融使得小鼠肿瘤抑制率达到100%。以新西兰白兔VX-2肝脏原位移植瘤为模型,经肝动脉超选择介入给药后,数字减影血管造影(DSA)图像显示肿瘤和周围血管迅速"消失",mPEG-PLGA微球具有良好的栓塞效果。微波消融患处,材料+微波组6天后肿瘤在两个方向的消融直径分别达到15.76和21.85mm(微波组为11.18和11.78mm)。本文开发的mPEG-PLGA微球实现了动脉栓塞与微波增敏消融的协同肿瘤治疗,可为原发性肝癌的治疗提供有效医用材料。

In this paper,mPEG-PLGA microspheres with microwave sensitivity and good biosafety were prepared by double emulsion method for the treatment of hepatocellular carcinoma.The microspheres showed a uniform monodisperse with an average size of about 63 jj.m.Compared with saline,the microspheres coated with M0S2 nanosheets dispersed in saline can be increased by 8.5°C under microwave irradiation.For the subcutaneous H22 tumor of ICR mice,the ablation rate of tumor was 100%under microwave irradiation.For VX-2 liver orthotropic transplantation tumor in New Zealand white rabbits,the DSA images showed that the tumor and peripheral blood vessels disappeared rapidly after the hepatic arterial embolization,indicating the microspheres had a good embolization effect.6 days after ablation,the necrosis diameters of the tumor in the two directions were 15.76 and 21.85 mm,respectively,compared with the microwave group(11.18 and 11.78 mm).