宫颈鳞状细胞癌组织中CD44v6、LYVE-1表达与患者临床病理特征及预后的关系

Relationships between expression of CD44v6 and LYVE-1 in cervical squamous cell carcinoma and clinicopathological features and prognosis of patients

目的探讨白细胞分化抗原44号拼接变异体v6(CD44v6)、人淋巴内皮透明质酸受体-1(LYVE-1)在宫颈鳞状细胞癌组织中的表达与患者临床病理特征及预后的关系。方法收集宫颈鳞状细胞癌患者宫颈组织78例份、宫颈鳞状上皮内病变患者宫颈组织47例份和正常宫颈组织32例份,免疫组化(SP法)检测各组宫颈组织中CD44v6、LYVE-1蛋白的表达。分析癌组织CD44v6、LYVE-1蛋白的表达与宫颈鳞状细胞癌患者临床病理特征的关系。绘制生存曲线,分析癌组织CD44v6、LYVE-1表达与宫颈鳞状细胞癌患者预后的关系。结果 CD44v6、LYVE-1蛋白表达比较:正常宫颈组织<宫颈鳞状上皮内病变宫颈组织<宫颈鳞状细胞癌宫颈组织(P均<0.05)。癌组织CD44v6、LYVE-1表达均与组织学分级、肌层浸润深度、淋巴管浸润及淋巴结转移等相关(P均<0.05),与患者年龄、肿瘤直径无关(P均>0.05); CD44v6的表达与临床FIGO分期有关(P<0.05)。CD44v6与LYVE-1表达呈正相关(rs=0.408,P=0.000)。与CD44v6、LYVE-1表达阴性患者比较,CD44v6、LYVE-1表达阳性患者生存率低、生存时间短(P均<0.05)。结论 CD44v6、LYVE-1在宫颈鳞状细胞癌组织中过量表达,且均与患者不良预后相关,二者可能协同参与病情进展。

Objective To explore the expression of CD44v6and lymphatic vessel endothelial hyaluronan receptor-1(LYVE-1)in cervical squamous cell carcinoma and its relationships with the patients’clinicopathologic features and prognosis.Methods Seventy-eight cervical specimens from patients with cervical squamous cell carcinoma,47specimens from patients with cervical squamous intraepithelial lesion and32specimens from normal cervical tissues were collected.Immunohistochemical method(SP)was used to detect the expression of CD44v6and LYVE-1protein in the cervical tissues of each group.We analyzed the relationships between the expression of CD44v6and LYVE-1protein and clinicopathological characteristics of cervical squamous cell carcinoma.The survival curve was drawn to analyze the relationship between the expression of CD44v6and LYVE-1and the prognosis of patients with cervical squamous cell carcinoma.Results The comparison of CD44v6and LYVE-1protein expression was in the following order:normal cervical tissues<squamous intraepithelial lesion tissues<cervical squamous cell carcinoma tissues(P<0.05).The expression levels of CD44v6and LYVE-1were closely related to the histological grading,myometrial invasion,lymphatic invasion and metastasis(all P<0.05),but not related to the age or the size of the tumor(both P>0.05);the CD44v6expression was related to FIGO stage(P<0.05).There was an positive correlation between the expression of CD44v6and LYVE-1(γ=0.408,P=0.000).The patients with positive expression of CD44v6or LYVE-1had lower survival rate and shorter survival time as compared with patients with negative expression(both P<0.05).Conclusion CD44v6and LYVE-1are overexpressed in the tissues of cervical squamous cell carcinoma,and are related to poor prognosis,which may be involved in the disease progression.