Box-Behnken Design响应面法结合遗传算法和直接搜索算法优化丹栀逍遥散的提取工艺

Optimization of Extraction Technology of Danzhi Xiaoyao Powder by Box-Behnken Design Response Surface Methodology Combined with Genetic Algorithm and Direct Search Methods

目的:优化丹栀逍遥散的提取工艺。方法:采用加热回流提取法提取丹栀逍遥散组方药材样品粉末,以乙醇体积分数、提取时间和液料比(V/m)为考察因素,以栀子苷、芍药苷和丹皮酚3个指标成分的综合评分为评价指标,先采用Box-Behnken Design(BBD)响应面法进行试验设计,建立数学模型并获得目标函数后,结合遗传算法和直接搜索算法求解各因素的最优取值。结果:丹栀逍遥散中3个指标成分的最优提取工艺条件为60.8%乙醇提取3.1 h、液料比为15.72∶1(mL/g)。采用BBD响应面法或结合遗传算法和直接搜索算法所得的综合评分比较差异无统计学意义(P>0.05)。结论:经优化的工艺可用于丹栀逍遥散中3个指标成分的提取。BBD响应面法结合遗传算法和直接搜索算法在优化中药提取工艺方面具有一定优越性。

OBJECTIVE:To optimize the extraction technology of Danzhi xiaoyao powder.METHODS:The medicinal powder in the formula of Danzhi xiaoyao powder was extracted by using the heating reflux method.Box-Behnken Design(BBD)response surface methodology(RSM)was adopted for trial design with ethanol volume fraction,extraction time and ratio of water to material(V/m)as factors by using comprehensive scores of geniposide,paeoniflorin and paeonol as evaluation indexes.After mathematical model and objective function were obtained,genetic algorithm and direct search method were used to obtain the optimum ranges and value of extraction process parameters.RESULTS:The optimal extraction technology of three indexes in Danzhi xiaoyao powder included 60.8%ethanol,extraction time of 3.1 h,water-material ratio of 15.72∶1(mL/g).There was no statistical significance in the difference of comprehensive scores by BBD RSM alone or combined with genetic algorithm and direct search method(P>0.05).CONCLUSIONS:The technology can be used for the extraction of index from Danzhi xiaoyao powder.BBD-RSM combined with genetic algorithm and direct search method has certain superiority in optimizing the extraction process of TCM.