乌蕨醇提取物对1型糖尿病大鼠的降血糖作用及其机制初探

Hypoglycemic Effects and Its Mechanism of Ethanol Extract from Stenoloma Chusanum(L) Ching on Type 1 Diabetic Rats Induced by Streptozotocin

为探讨乌蕨醇提取物对1型糖尿病大鼠的降血糖作用及其机制,本研究以链脲佐菌素(STZ)腹腔注射诱导建立1型糖尿病大鼠模型,并将大鼠分为空白对照组、1型糖尿病模型组、乌蕨醇提取物低剂量组(30 mg/kg)和高剂量组(60 mg/kg)。分别用生理盐水及乌蕨醇提取物每天灌胃1次,连续28 d,灌胃容积为20 m L/kg。结果证明,乌蕨醇提取物可减缓STZ致1型糖尿病大鼠体重的负增长,降低空腹血糖水平(P <0. 05);升高胰岛素及葡萄糖激酶(GCK)含量;降低醛糖还原酶(AR)含量(P <0. 05)。同时,胰腺组织HE染色结果显示:乌蕨提取物高、低剂量组糖尿病大鼠的胰岛数目较1型糖尿病模型组显著增多,胰岛及外分泌腺萎缩均有不同程度减轻。提示乌蕨提取物可显著降低STZ致1型糖尿病模型大鼠空腹血糖水平,其降糖作用可能与升高糖尿病大鼠血清葡萄糖激酶含量和降低AR含量、改善糖尿病大鼠胰岛损伤、促进胰岛β细胞分泌胰岛素有关。

To investigate the hypoglycemic effects of ethanol extract and its mechanism from Stenoloma chusanum(L)Ching on type1diabetic rats.The research group established a model of type1diabetes rats by intraperitoneal injection of streptozotocin(STZ).The SD rats were randomly divided into four groups,including control group,model group,low dose group of30mg/kg(LG)and high dose group of60mg/kg(HG)and diabetic rat’s model induced by STZ.Rats in LG and HG was oral administration by ethanol extract from SLC once a day until28days and gastric perfusion volume is20mL/kg.The body weight,fasting blood glucose(FBG),serum insulin(INS),glucokinase(GCK)and aldose reductase(AR)of rats in each group were measured and the morphology of pancreas was observed The28days igadministration of extract of SLC decreased the negative growth of body weight,FBG and AR significantly in diabetic rats induced by STZ(P<0.05)and increased IINS,GCK(P<0.05).Morphological observations show that the number of islets in LG and HG was more than that in model group.Islet and exocrine atrophy were reduced in varying degrees.Ethanol extract from SLC has the hypoglycemic effect on fasting blood glucose in diabetic rats induced by STZ,and the mechanism may be related to decreasing aldose reductase,increasing serum glucokinase,protecting the islets and promoting insulin secretion by pancreaticβcells.