蓝影与四甲基联苯胺显现潜血鞋印的比较研究

Comparison between BlueBlood and TMB on Detection of Latent Blood Footprint

蓝影是一种新型的潜血印痕增强试剂,探究它的显现效果、适用场景和注意事项有助于提高其在实战中的应用效果。根据蓝影的特点,笔者设计实验分析比较了蓝影和四甲基联苯胺对油漆木地板、瓷砖、水磨石、大理石和地板革等非渗透性客体上潜血鞋印的显现效果。在这些潜血鞋印的常见承痕客体上,蓝影和四甲基联苯胺对不同浓度潜血鞋印都有较好的显现效果,但蓝影总体显现效果优于四甲基联苯胺,显现效果更清晰,无扩散及背景模糊现象,且不影响酸性黄等荧光试剂的二次显现。研究发现,牛奶和鸡蛋清等会使蓝影出现一定程度假阳性的反应,使用过程中要注意避免假阳性反应干扰染色效果。

BlueBlood,a new type of reagent for enhancement of latent blood trace,was here explored of its developing effect,available scenes and attentions necessary to be paid so as to improve its functions in actual crime-combating.An experiment was thus designed to compare the effect of BlueBlood and TMB(tetramethyl benzidine)on highlighting the latent blood footprints on such non-osmotic objects as painted wood floors,tiles,terrazzo,marble and floor leather.On the indicated-above common objects carrying latent blood footprints,both BlueBlood and TMB showed good effects to unveil the footprints resulted from smearing different concentrations of blood,yet BlueBlood being overall better than TMB with its showing footprints clearer,nondiffuse and distinct background.Additionally,BlueBlood has no influence on the secondary development by fluorescent reagents,e.g.,acid yellow.Nevertheless,stuffs like milk and egg white can cause a certain degree of false positive reaction to BlueBlood development,therefore attention should be paid to avoid the interference from false positive reaction.