高效液相色谱梯度洗脱法同时测定调气丸中6个主要成分含量

Simultaneousdetermination of six main components in Tiaoqi Wan by HPLC gradient elution method

目的建立调气丸中和厚朴酚、厚朴酚、牡荆素葡萄糖苷、牡荆素鼠李糖苷、牡荆素和金丝桃苷含量的测定方法。方法采用Venusil MP C_(18)色谱柱(200 mm×4.6 mm,5μm),流动相:乙腈—1.0%冰醋酸溶液,梯度洗脱,流速:0.9 mL/min,柱温30℃。结果和厚朴酚、厚朴酚、牡荆素葡萄糖苷、牡荆素鼠李糖苷、牡荆素和金丝桃苷分别在2.97～59.40、4.26～85.20、3.59～71.80、20.68～413.60、2.66～53.20、1.84～36.80 mg/L范围内线性关系良好;平均回收率分别为97.95%(RSD=0.98%)、98.27%(RSD=1.26%)、 98.19%(RSD=1.15%)、99.96%(RSD=0.68%)、 97.88%(RSD=1.68%)、96.93%(RSD=1.24%);精密度和重复性良好;供试品溶液在室温条件下12 h内稳定。结论该方法操作简便,精密度、稳定性、重复性好,可同时用于调气丸中6种主要成分的含量测定。

Objective To establish a method for the determination of honokiol,magnolol,vitexin-4'-O-glucoside,vitexin-2'-o-rhamnoside,vitexin and hyperoside in Tiaoqi Wan.Methods The chromatographic column was Venusil MP C 18(200 mm×4.6 mm,5μm),using the gradient mobile phase composed of acetonitrile 1.0%glacial acetic acid solution.The flow rate was 0.9 mL/min,and the temperature of column was 30℃.Results The linear ranges of honokiol,magnolol,vitexin-4'-O-glucoside,vitexin-2'-o-rhamnoside,vitexin and hyperoside were within 2.97 to 59.40,4.26 to 85.20,3.59 to 71.80,20.68 to 413.60,2.66 to 53.20,and 1.84 to 36.80 mg/L with average recoveries of 97.95%(RSD=0.98%),98.27%(RSD=1.26%),98.19%(RSD=1.15%),99.96%(RSD=0.68%),97.88%(RSD=1.68%),and 96.93%(RSD=1.24%).The precision and repeatability were good.Test solution was stable at room temperature within 12 h.Conclusions The method is simple with good precision,stability and reproducibility,and it can be used for the simultaneous determination of 6 components inTiaoqi Wan.