5-脂氧合酶抑制剂Zileuton缓解压力负荷诱导的心肌重构

Effect of Zileuton on Cardiac Remodeling Induced by Pressure-overload in Mice

目的探讨5-脂氧合酶抑制剂Zileuton对压力负荷诱导小鼠心肌重构和心功能的影响。方法部分结扎C57小鼠胸主动脉制作小鼠压力负荷诱导的心肌重构模型,并在术后1周Zileuton灌胃干预,每天1次,每次70mg/kg,观察至术后7周,行心脏超声检测后取材,称取小鼠体重、心重和肺重,并留取病理标本,行左心室心肌组织HE和天狼星红染色。结果 Zileuton抑制了小鼠心肌重构和心功能下降,包括改善了射血分数,抑制了左心室舒张末内径、室间隔厚度、左心室后壁厚度的增加,减轻了心肌细胞肥大和心肌组织胶原沉积。结论 Zileuton能够抑制压力负荷诱导的小鼠心肌重构,5-LOX可能成为治疗心力衰竭的潜在靶点。

Objective To investigate the effect of Zileuton on cardiac remodeling induced by pressure-overload in mice.Methods Male,8-10-week-old,wild-type C57 mice with or without oral zileuton administration were subjected to aortic banding(AB)or a sham operation.Results Our data demonstrated that Zileuton protected against cardiac remodeling and dysfunction induced by AB,as assessed by heart weigh/body weight,lung weight/body weight,echocardiographic parameters,and histological analysis.Conclusion These findings suggest a potential therapeutic target of 5-LOX for cardiac remodeling and heart failure.