DNA去甲基化酶对拟南芥Laccase家族基因甲基化及转录模式的影响

Effects of DNA demethylase on the methylation and transcriptional patterns of the Arabidopsis Laccase genes

植物Laccase家族蛋白,是铜蓝氧化酶超家族的重要成员,具有包括抗病性在内的多种生物学功能。利用拟南芥野生型及ros1、rdd和dme突变体的全基因组甲基化测序(BS-SEQ)数据,分析了拟南芥Laccase蛋白家族基因(AtLACs)的DNA甲基化模式。结果表明:在野生型和不同DNA去甲基化酶突变体中,该家族少数成员在基因上游区和转录区存在较高水平的甲基化修饰现象。转录组测序(RNA-SEQ)数据分析结果显示,在野生型和不同突变体中,ALAC家族基因的转录水平普遍较低。而AT5G48100基因在拟南芥胚乳细胞中剧烈表达,DME突变后,其表达水平进一步剧烈增强。综合而言,在野生型和不同DNA去甲基化酶突变体中,ALAC基因家族大部分成员的DNA甲基化修饰与转录调控关联不强,但有些基因的DNA甲基化程度与转录水平之间有密切联系。

Plant laccase family belongs to the important members of multicopper oxidases,which constitute a superfamily.Laccases in plants play several roles including disease resistance.Here,we analyzed the DNA methylation pa-terns and transcriptional levels of the Arabidopsis thaliana laccase-encoding genes(AtLACs)in samples from a wild type(Col-0)and ros1,rdd,and dme mutants.Our results showed that a few of the family members in Col-0,ros1,rdd,and dme mutants were highly methylated within the upstream and the gene body regions.On the other hand,most of the ALAC genes were expressed at low levels in the wild type and the three mutants.However,AT5G48100,which encodes a laccase in A.thaliana,was found to be highly expressed in Arabidopsis endosperm cells.And the mutation of DME increased strikingly the transcriptional level of AT5G48100.The results indicated that the DNA methylation levels of most AtLACs were not strongly correlated to their transcriptional levels,whereas the close relationships were observed between the methylation and transcription in individual AtLACs.