金针菇子实体多糖FVPB2对小鼠T淋巴细胞和巨噬细胞的免疫调节作用

Immunomodulatory effect of homogeneous polysaccharide FVPB2 from fruiting bodies of Flammulina filiformis on T lymphocytes and macrophages of mice

从金针菇子实体中分离纯化得到均一多糖FVPB2,其分子量为15kDa,是由葡萄糖、半乳糖、岩藻糖和甘露糖组成的吡喃型杂多糖,利用C57BL/6小鼠脾淋巴细胞和骨髓巨噬细胞研究FVPB2对免疫功能的影响,体外免疫实验表明,FVPB2能促进T淋巴细胞激活并分泌肿瘤坏死因子和干扰素γ细胞因子,同时还能够促进巨噬细胞产生一氧化氮,分泌白介素-1β、白介素-6和肿瘤坏死因子-α细胞因子。本研究以首次从金针菇子实体中获得均一多糖FVPB2为研究对象,观察其对T细胞和巨噬细胞的免疫调节活性,研究结果表明其具有良好的免疫调节活性和潜在的生物学功能。

The homogeneous polysaccharides FVPB2 from the fruiting body of Flammulina filiformis were separated and purified for the first time.The purified polysaccharide was a heteropolysaccharide consisting of D-glucose,D-galactose,L-fucose,and D-mannose,with an average molecular weight of about 1.5×10~4Da.The immunoactivity of FVPB2 in vitro was evaluated on spleen lymphocyte and bone marrow macrophage in C57BL/6 mice.In vitro immunomodulatory studies showed FVPB2 induced T lymphocyte activation,and stimulated TNF and IFN-γsecretion of T lymphocyte.FVPB2 also could induce NO production and IL-1β,IL-6and TNF-αsecretion of bone marrow macrophage.It was proved that the homogenous polysaccharide FVPB2could enhance non-specific immune response and specific immune responses in vitro.