与内生真菌共培养对葡萄果肉细胞生理生化影响的差异研究

Differentially Physiological and Biochemical Responses of Grape Cells to Contacted Co-culture Endophytic Fungal Strains

根据与内生真菌共培养过程中对葡萄细胞生理生化的影响差异将内生真菌菌株归类,筛选出具有不同利用价值的内生真菌资源。以分离于玫瑰蜜、赤霞珠和夏黑葡萄叶片的18个属47株内生真菌和佳美葡萄(Vitis vinifera L.cultivar:Gamay)果肉愈伤组织为试验材料,构建内生真菌与葡萄细胞共培养体系,并探索这些内生真菌菌株对葡萄果肉细胞生长、花色苷含量、苯丙氨酸解氨酶(PAL)活性等的生理生化影响。结果表明,与不同内生真菌共培养对葡萄细胞生长、花色苷总量和PAL活性都有不同程度的影响。其中共筛选出20株对葡萄细胞伤害较小的内生真菌;6株显著促进葡萄细胞生长的内生真菌;11株显著促进葡萄细胞PAL活性的内生真菌;5株促进葡萄细胞花色苷总量的内生真菌。此次研究为发掘和利用内生真菌资源调控葡萄生化品质特征提供了技术参考和一定的物质基础。

Endophytic fungal strains were classified according to the differentially physiological and biochemical responses of grape cells during their co-culturing with endophytic fungi,which may be conducive to screening endophytic fungal resources with varied application values. Grape callus(Vitis vinifera L. cultivar :Gamay)and 47 endophytic fungal strains from 18 genera isolated from grape varieties Rose honey,Cabernet Sauvignon and Xiahei were chosen as experimental materials to construct endophytic fungi-grape cell contacted co- culture system. Further the physiological and biochemical effects of these endophytic fungal strains on grape cellular,growth,anthocyanin contents,phenylalanine ammonia-lyase(PAL)activities and others were analyzed. Ours results indicated that different endophytic fungal strains initiated different degrees of impacts on the co-cultured grape cells in growth,anthocyanin contents and PAL activity. Among all those used endophytic fungal strains,20 conferred less damages to grape cells,6 significantly promoted the co-cultured grape cells in growth,11 significantly promoted the co-cultured grape cellular PAL activity,and 5 promoted the co-cultured grape cells’ anthocyanin contents. Beside the development of candidate endophytic fungal strains,this research provided a methodology for purposely screening candidate endophytic fungal resources.