地肤子总黄酮对二硝基氯苯致大鼠接触性皮炎的抗炎作用

Anti-inflammatory effect of the total flavonoids of Fructus Kochiae in DNBC-induced allergic contact dermatitis rats

目的研究地肤子总黄酮(FFK)对二硝基氯苯(DNBC)致大鼠变态反应性接触性皮炎(ACD)的抗炎作用。方法SPF级SD大鼠40只,雌雄各半,按随机数字表法分为五组,每组8只,即空白对照组、ACD模型组、阳性药对照组(醋酸泼尼松龙2.5mg/kg)、FFK低剂量组(75mg/kg)、FFK高剂量组(150mg/kg);以DNBC建立大鼠ACD模型后,予以FFK灌胃给药,连续10d。观察皮肤变态性反应和肿胀度,同时测定血清中白细胞介素-6(IL-6)、白细胞介素-10(IL-10)和γ干扰素(IFN-γ)表达水平,并通过免疫组化检测耳组织中核因子κBp65(NF-κBp65)表达水平。结果与空白对照组比较,ACD模型组大鼠皮肤肿胀度明显增高,血清中IL-6、IFN-γ含量与耳组织中NF-κBp65蛋白表达明显增高,而血清中IL-10含量明显降低(P<0.01);与ACD模型组比较,FFK低剂量组与FFK高剂量组大鼠的耳肿胀度、血清中IL-6、IFN-γ含量、耳组织中NF-κBp65蛋白表达水平显著降低(P<0.01),而血清中IL-10含量显著升高(P<0.01)。结论FFK对于DNBC致大鼠ACD具有抗炎作用,其机制可能是通过抑制NF-κBp65蛋白发挥作用。

Objective To investigate the anti-inflammatory effect of the total flavonoids of Fructus Kochiae (FFK) on DNBC-induced allergic contact dermatitis (ACD) rats.Methods According to random number table method,40 half male and half female SPF grade SD rats were randomly divided into 5 groups,with 8 rats in each group,including blank control group,ACD model group,positive control group (Splash Methylprednisolone 2.5 mg/kg),FFK low-dose group (75 mg/kg),FFK high-dose group (150 mg/kg).After ACD model were established by treatment of DNCB on right ear for 10 days,rats were treated with FFK in 10 d and the allergic reaction and swelling degree of ears were observed,the content of IL-6,IL-10 and IFN-γ in serum and the level of NF-κB p65 in ears were determined.Results Compared with the blank control group,the swelling of ears in ACD model group was significantly increased,the content of IL-6,IFN-γ in serum and the level of NF-κB p65 in ears were up-regulated,while the content of IL-10 was down-regulated in serum (P < 0.01).Compared with the ACD model group,the ears swelling degree of rats in FFK low-dose group and FFK high-dose group was significantly decreased,the content of IL-6,IFN-γ in serum and the level of NF-κB p65 in ears were significantly down-regulated,while the content of IL-10 in serum was significantly up-regulated (P < 0.01).Conclusion FFK has anti-inflammatory effect on DNBC-induced ADC rats and its mechanism involved in down-regulate the NF-κB p65.