梅花鹿茸血冻干粉的安全性评价及缓解体力疲劳功能研究

Safety evaluation of freeze-dried powder of sika deer antler blood and its effect on alleviating physical fatigue in mice

通过小鼠急性毒性试验,大鼠30d喂养试验,三项遗传毒性试验,小鼠尾部铅皮负重游泳,小鼠血清中尿素水平检测,小鼠肝脏中糖原储备量测定以及小鼠运动前后血清中乳酸水平的测定对梅花鹿茸血冻干粉的食品安全性及对小鼠的缓解体力疲劳作用进行探讨。结果表明:小鼠急性经口毒性>15g/kg·BW,属无毒级;鹿茸血冻干粉0.6,1.3,2.0g/(kg·BW·d)剂量在30d喂养试验中未对大鼠的体重、摄食量、血液常规和血液生化指标产生影响,且三项遗传毒性试验结果均为阴性。0.6g/(kg·BW·d)组小鼠负重游泳时间长于阴性对照组,差异有统计学意义(P <0.05);0.1,0.2,0.6g/(kg·BW·d)组肝糖原储备量高于阴性对照组,差异有统计学意义(P<0.05);0.6g/(kg·BW·d)组小鼠游泳前后血乳酸变化的曲线下面积值低于阴性对照组,差异有统计学意义(P<0.05)。梅花鹿茸血冻干粉食用安全,且具有缓解小鼠体力疲劳功能。

To evaluate the food safety of sika deer antler blood freeze-dried powder,the acute toxicity test in mice,and the 30-day feeding and three genotoxicity tests in rats were conducted.The effects of sika deer antler blood freeze-dried powder on alleviating physical fatigue in mice were studied by weight-bearingswimming in the tails,determination of the urea level in serum,the glycogen reserve in liver and the lactic acid level in serum before and after the exercises.Results:the freeze-dried powder of sika deer antler blood was a substance without toxicity according to the acute toxicity test(MTD>15 g/kg·BW).The dosage0.6,1.3,and 2.0 g/(kg·BW·d)of freeze-dried powder of sika deer antler blood did not affect the body weight,food intake,blood routine and blood biochemical indexes of rats in 30 days feeding test.The results of three genotoxicity tests were negative.The time of weight-bearing swimming of 0.6 g/kg·BW ·d)group was longer than that of the negative control group.The hepatic glycogen reserve of 0.1,0.2,and 0.6 g/(kg·BW ·d)groups was higher than that of the negative control group.The area under the curve of blood lactate change in 0.6 g/(kg·BW·d)group was lower than that in the negative control group.These differences have a statistical significance(P<0.05).Conclusion:The freeze-dried powder of sika deer antler blood was safe to eat and had the function of alleviating physical fatigue.