期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

错配杂交化学发光法检测细胞色素P4501A1基因多态性 被引量：3

Chemiluminescence Detection of Cytochrome P4501A1 Polymorphisms by Mismatch Hybridization
下载PDF
导出
摘要 建立一种检测细胞色素P4 5 0 1A1基因多态性的新方法。针对每个多态位点设计两根寡核苷酸探针 ,两根探针的区别仅在于突变位点处一个碱基。每个样品分别与两根探针进行杂交 ,采用化学发光法检测杂交体 ,通过两根探针的杂交信号强度之比确定基因型。与参考方法比较 ,该法具有快速、简便。 An assay for the detection of cytochrome P4501A1 (CYP1A1) polymorphisms has been developed, Two oligonucleotide probes for each polymorphic site differing only, in a single nucleotide at mutant site were designed. Each sample was hybridized with two probes distinctively and the hybrids were detected by chemiluminescence. The genotype was identified by calculating the ratio of signals obtained with two probes. Compared with reference method, this assay was rapid, easy to operate and economical.
作者 吴晓明 周宜开 任恕 郝巧玲
机构地区 华中科技大学同济医学院环境医学研究所
出处 《分析化学》 SCIE EI CAS CSCD 北大核心 2002年第11期1355-1358,共4页 Chinese Journal of Analytical Chemistry
基金 国家自然科学基金资助项目 (No .39990 5 70 )
关键词 错配杂交 化学发光法 检测 细胞色素P4501A1 基因多态性 肺癌 mismatch hybridization chemiluminescence cytochrome P4501A1 genetic polymorphisms
分类号 R734.2 [医药卫生—肿瘤] R446.19 [医药卫生—诊断学]
  • 相关文献

参考文献7

  • 1[1]Raunio H, Husgafvel-Pursiainen K, Anttila S. Gene.,1995,159(1):113～121
  • 2[2]Kawajiri K, Eguchi H, Nakachi K. Cancer Research,1996,56:72～76
  • 3[3]Chen S, Xue K, Xu L. Mutation Research,2001; 458:41～47
  • 4[4]Stucher I, Jacquet M, de Waziers. Pharmacogenetics,2000,10:617～27
  • 5[6]Song N, Tan W, Xing D Y. Carcinogenesis,2001,22:11～16
  • 6[7]Klingler K R, Junold T, Wielckens K. Clinical Chemistry,1999,45:1925～1931
  • 7[8]Ikuta S, Takagi K, Wallace R B. Nucleic Acids Research,1987,15:797～811

同被引文献92

  • 1秦民坚,刘俊,吉文亮,赵俊,丁家宜,余国奠.生物化学发光法测定射干类中药清除自由基的作用[J].药学实践杂志,2000,18(5):304-306. 被引量：24
  • 2武竟存,章竹君,吕九如.液相色谱化学发光检测法的新进展[J].分析化学,1994,22(4):396-405. 被引量：9
  • 3[1]a.Dodeigne C,Thunus L,Lejeune R.Chemiluminescence as diagostic tool.A review[J].Talanta,2000,51(3):415-439.b.Stigbrand M,Ponten E,Irgum K.1,1′-Oxalyldiimidazole as chemiluminescence reagent in the determination of low hydrogen peroxide concentration by flow injection analysis[J].Anal.Chem.,1994,66(10):1766-1770.c.Barnett N W,Bos R,Lews S W.Rational design and preliminary analytical evalution of two novel oxamide reagents for aqueous peroxyoxalate chemiluminescence[J].Analyst,1998,123(6):1239-1244.
  • 4[6]Nau V,Nieman T A.Application of microporous membranes to chemiluminescence analysis[J].Anal.Chem.,1979,51(3):424-428.
  • 5[7]Sigvardson K W,Kennish J M,Birks J W.Peroxyoxalate chemiluminescence detection of polycyclic aromatic in liquid chromatography[J].Anal.Chem.,1984,56(7):1096-1102.
  • 6[8]Piet V Z,Kamminga D A,Gooijer C,et al.Quenched peroxyoxalate chemiluminescence as a new detection principle in flow injection analysis and liquid chromatography[J].Anal.Chem.,1986,58(6):1245-1248.
  • 7[9]Kamtekar S D,Pande R,Ayyagari M S,et al.Trace analysis of Zn(Ⅱ),Be(Ⅱ),and Bi(Ⅲ) by enzyme-catalyzed chemiluminescence[J].Anal.Chem.,1996,68(1):216-220.
  • 8[10]He Zike,Gao Hua,Yuan Liangjie,et al.Simultaneous determination of oxalic and tartaric acid with chemiluminescence detection[J].Analyst,1997,122(11):1343-1345.
  • 9[11]Gamborg G,Hansen E H.Flow-injection bioluminescent determination of ATP based on the use of the luciforin-luciferase system[J].Anal.Chim.Acta,1994,285(3):321-328.
  • 10[21]a.Qin Wei,Zhang Zhujun,Liu Huajun.Chemiluminescence flow-through sensor for copper based on an arodic stripping voltammetric flow cell and an ion-exchange column with immobileized reagents[J].Anal.Chem.,1998,70(17):3579-3584.b.Zhang Chengxiao,Zhang Xichun,Zhang Zhujun.Determination of copper ions in waters by electrochemical stripping chemiluminescence analysis[J].Analyst,1998,126(6):1383-1386.

引证文献3

二级引证文献9

分析化学
2002年 第11期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部