P21 WAF_1/CIP_1在卵巢上皮性肿瘤中的表达与临床意义的研究

Study on expression and clinical significance of P21 WAF_1/CIP_1 in epithelial overian neoplasms

目的 :探讨P2 1WAF1/CIP1在恶性卵巢上皮性肿瘤中的表达与临床意义。方法 :应用免疫组化S P检测P2 1WAF1/CIP1在 2 0例良性、10例交界性、5 5例恶性卵巢上皮性肿瘤中的表达 ,并分析P2 1WAF1/CIP1与恶性卵巢上皮性肿瘤临床病理指标的关系。结果 :P2 1WAF1/CIP1在良性、交界性与恶性卵巢上皮性肿瘤中的表达总阳性率及强阳性率均有显著性差异 (P =0 0 0 7,P强 =0 0 0 1) ,并随良性、交界性、恶性而降低表达 (P <0 0 1,P强 <0 0 0 1) ;与恶性卵巢上皮性肿瘤的临床分期、组织分化、淋巴转移、腹水及预后均有关 (P =0 0 11,0 0 0 0 ,0 0 14 ,0 0 11;P强 =0 0 37,0 0 0 3,0 0 16 ,0 0 0 2 ,P <0 0 5 )。结论 :P2 1WAF1/CIP1在恶性卵巢上皮性肿瘤的形成和发展中发挥重要作用 ,检测P2 1WAF1/CIP1蛋白表达情况对卵巢肿瘤的良恶性鉴别 ,判断预后 ,探索卵巢癌的发生过程 ,从而指导卵巢癌的基因治疗均有积极的意义。

Objective:To study on the effects of the gene expression of P21WAF 1/CIP 1 oncogenesis and progression of epithelial ovarian neoplasms.Methods:The expression of P21WAF 1/CIP 1 protein in 20 cases of benign ovarian tumor, 10 cases of borderline ovarian tumor and 55 cases of epithelial ovarian carcinoma were analyzed by immunohistochemistry technique. The relationships between the results and clinicpathological features were studied.Results:Both the total positive and the strong positive expression for P21WAF 1/CIP 1 in benign, borderline and malignant epithelial ovarian tumors were obvious difference ( P =0 007, P strong =0 001) and decreased with development of ovarian tumors ( P <0 01, P strong <0 001). The expression of P21WAF 1/CIP 1 in epithelial ovarian neoplasms was associated with clinical stage,histologic grade,lymphonode metastasis,ascites and prognosis ( P =0 011,0 000,0 014,0 011; P strong =0 037,0 003,0 016,0 002, P <0 05).Conclusion:The experiment suggests that P21WAF 1/CIP 1 play important roles in oncogenesis and progression of epithelial ovarian cancers. Detection of P21WAF 1/CIP 1 gene proteins has positive effects on malignancy determination,prognosis and gene treatment of ovarian cancers.