过氧化氢法改性羊肚菌多糖研究

Study on modification of Morchella polysaccharide by hydrogen peroxide

为提高羊肚菌多糖体外降血糖能力,本试验在筛选羊肚菌多糖改性方法的基础上,以α-淀粉酶抑制率为参数,运用单因素试验结合响应面分析法建立了羊肚菌多糖改性产物对α-淀粉酶抑制率的多元二次回归方程模型,优化了羊肚菌多糖过氧化氢氧化降解工艺条件。试验表明料液比为1∶8.55、pH为7.09、温度为47.8℃、时间为3.02 h的条件下的改性效果最好,在该条件下重复测定三次所得羊肚菌多糖过氧化氢氧化改性产物的α-淀粉酶抑制率为16.30%±1.22%,与模型预测结果的相对误差为0.11%,是改性前的17.16倍。同时,蔗糖酶抑制率为78.13%±5.09%,麦芽糖酶抑制率为16.48%±3.27%,α-葡萄糖苷酶抑制率为21.40%±3.81%,分别比改性前提高了1.07倍、1.64倍、1.22倍。与常用的降糖药阿卡波糖相比,羊肚菌多糖改性产物的α-淀粉酶抑制率、麦芽糖酶抑制率、α-葡萄糖苷酶抑制率分别提高了1.44、1.63和1.88倍,蔗糖酶抑制率与阿卡波糖差异不显著(P>0.05)。羊肚菌多糖经过氧化氢氧化改性的产物呈现明显的多糖红外吸收特征,对糖苷酶抑制效果显著提升,提高了其体外降血糖能力。

To improve the hypoglycemic ability of Morchella polysaccharide in vitro,α-Amylase inhibition rate was detected with single factor test and response surface methodology.The multiple regression equation model ofα-amylase inhibition rate of Morchella polysaccharide modified products was also established.The predicted model indicates that the optimum reaction conditions are as follows:material-liquid ratio is 1∶8.55,pH is 7.09,and the reaction time is 3.02 h at 47.8℃.Under the same conditions,three parallel experimental results indicate that theα-amylase inhibition rate of the modified product of hydrogen peroxide oxidation of Morchella polysaccharide is 16.30%±1.22%,and the relative error between experiment and prediction of the model is 0.11%.Theα-amylase inhibition rate is17.16 times of that of the unmodified.In this case,the inhibition rates of sucrase,maltase andα-glucosidase were 78.13%±5.09%,16.48%±3.27%and 21.40%±3.81%respectively,which were 1.07,1.64 and 1.22 times higher than those before modification.Compared with acarbose,theα-amylase inhibition rate,maltase inhibition rate andα-glucosidase inhibition rate of the modified product of Morchella polysaccharide increased by 1.44,1.63 and 1.88 times respectively,while the sucrase inhibition rate was not significantly different from acarbose(P>0.05).The product modified by hydrogen peroxide oxidation of Morchella polysaccharide shows infrared absorption characteristics of polysaccharide,and significantly improves the inhibitory effect on glycosidase,and improves its hypoglycemic ability in vitro.