儿茶酚和莨菪碱对水稻纹枯病菌氨基酸合成和分泌的影响

Effects of Catechol and Hyoscyamine on Amino Acid Synthesis and Secretion in Rhizoctonia solani AG-1 IA

【目的】为明确水稻纹枯病菌(Rhizoctonia solani AG 1-IA)黑色素形成前体物质酪氨酸等氨基酸成份在不同培养处理中的差异。【方法】利用氨基酸分析仪对水稻纹枯病菌在3种培养处理(PDB对照,添加了300μg/mL质量浓度莨菪碱的PDB和50μg/mL质量浓度儿茶酚的PDB)后菌丝体和发酵液的氨基酸成份进行了分析。【结果】水稻纹枯病菌菌丝体中谷氨酸、天冬氨酸和丙氨酸3种氨基酸含量相对比较丰富,而谷氨酸的含量最高,达到总氨基酸的15.49%;水稻纹枯病菌发酵液中天冬氨酸和谷氨酸含量最为丰富,分别达到了总氨基酸的17.83%和58.21%;在3种处理中,不管是菌丝体还是发酵液中,都是谷氨酸和天冬氨酸最为丰富。影响水稻纹枯病菌菌丝体黑色素形成的前体物质酪氨酸的含量在3种处理的菌丝体中没有显著差异;但300μg/mL质量浓度的莨菪碱处理组与对照组和儿茶酚处理组中的苯丙氨酸含量都存在显著差异,而对照组和50μg/mL质量浓度的儿茶酚处理组的苯丙氨酸含量差异不显著。水稻纹枯病菌发酵液中对照组和50μg/mL质量浓度的儿茶酚处理组酪氨酸含量没有显著差异,但是300μg/mL质量浓度的莨菪碱处理组与对照组和儿茶酚处理组酪氨酸含量都存在显著差异,而苯丙氨酸在发酵液中差异不显著。【结论】影响水稻纹枯病菌菌丝体和发酵液黑色素形成的氨基酸不同,苯丙氨酸对水稻纹枯病菌菌丝体黑色素形成更加重要,而酪氨酸对水稻纹枯病菌发酵液黑色素形成更加重要,以上结果为氨基酸对水稻纹枯病菌黑色素形成的影响提供了参考依据。

[Objective]This study aimed to clarify the difference in amino acid composition such as tyro⁃sine,the precursor of melanin synthesis of Rhizoctonia solani AG-1 IA in different culture treatments[.Method]The amino acid compositions of mycelia and fermentation broth of R.solani AG-1 IA after three culture treat⁃ments(PDB control,PDB supplemented with 300μg/mL hyoscyamine and PDB supplemented with 50μg/mL catechol)were analyzed by amino acid analyzer.[Result]The contents of 3 kinds of amino acids,i.e.glutamic acid,aspartic acid and alanine in the mycelia of R.solani AG-1 IA were relatively abundant,among them,the content of glutamic acid was the highest,reaching 15.49%of the total amino acid.The contents of aspartic acid and glutamic acid in the fermentation broth of R.solani AG-1 IA were the most abundant,reaching 17.83%and 58.21%of the total amino acid,respectively.Glutamic acid and aspartic acid were the most abundant in mycelia and fermentation broth of R.solani AG-1 IA.The content of precursor tyrosine of melanin formation in mycelia had no significant difference in the comparison of the three mycelia of R.solani AG-1 IA;however,the content of precursor phenylalanine of melanin formation in 300μg/mL hyoscyamine treatment had signifi⁃cant differences from that in the control and the catechol treatment,while the control and the 50μg/mL cate⁃chol treatment had no significant difference.The tyrosine contents of the control and the 50μg/mL catechol treatment of R.solani AG-1 IA in fermentation liquid had no significant differences,but that in the 300μg/mL hyoscyamine treatment had significant difference from that in the catechol treatment and the control,while no significant difference was found in the fermentation liquid of phenylalanine[.Conclusion]The amino acids affect⁃ing the melanin formation of R.solani AG-1 IA mycelia and fermentation broth are different.Phenylalanine is more important for the melanin formation of R.solani AG-1 IA mycelia,while tyrosine is more important for the melanin formation of fermentation broth of R.solani AG-1 IA,these results provide a reference for the effect of amino acids on melanin formation of Rhizoctonia solani AG-1 IA.