牙龈卟啉单胞菌对db/db小鼠骨髓来源巨噬细胞极化作用的初步探究

A preliminary study of polarization of bone marrow-derived macrophages stimulated by porphyromonas gingivalis in db/db mice

目的初步探讨牙龈卟啉单胞菌(Pg)对db/db小鼠骨髓来源的巨噬细胞(BMDMs)极化的影响。方法以Pg刺激野生型C57BL/6J及db/db小鼠BMDMs,检测M1、M2基因标志物的表达水平。采用qRT-PCR法分别检测Tnf-α、Il-12以及Arg1、Ym1、Fizz1和Mrc1的基因相对表达量。结果与野生型相比,在IL-4诱导下,Pg感染与否对db/db小鼠BMDMs表达基因Arg1、Fizz1和Mrc1的影响无统计学差异;db/db小鼠BMDMs中Ym1的表达明显下降,M1极化标志基因Tnf-α无明显差异,Il-12的表达显著降低。结论在db/db小鼠中Pg诱导M2向M1转变的促进作用减弱。

Objective To investigate the effects of porphyromonas gingivalis(Pg) on polarization of bone marrow-derived macrophages(BMDMs) in db/db mice. Methods Pg was used to stimulate BMDMs from wild type C57 BL/6 or db/db mice and the the expression level of M1 and M2 gene markers was detected. The relative expression levels of Tnf-α, Il-12, Arg1, Ym1, Fizz1 and Mrc1 were detected by qRT-PCR. Results Compared with wild type mice, there was no statistically significant difference in the effect of Pg infection or not on BMDMs expressing Arg1, Fizz1 and Mrc1 in db/db mice under IL-4 induction;however, the expression of Ym1 in BMDMs of db/db mice significantly decreased, while no significant difference was found in Tnf-α, and the expression of Il-12 markedly decreased. Conclusion The ability of Pg to switch from M2 to M1 is weakened in BMDMs of db/db mice.