制何首乌的炮制方法探索

Exploring Research on Processing Method of Polygoni Multiflori Radix Praeparata

目的:考察黑豆汁炖制时间对制何首乌外观性状及各类有效成分含量变化规律的影响。方法:采用HPLC同时测定不同炮制时间制何首乌样品中二苯乙烯苷、大黄素、大黄素甲醚、大黄素-8-O-β-D-葡萄糖苷、大黄素甲醚-8-O-葡萄糖苷的含量,选择Agilent ZORBAX Extend-C18色谱柱(4.6 mm×250 mm,5μm),甲醇(A)-水(B)为流动相梯度洗脱(0~30 min,5%~100%A;30~40 min,100%A),流速1.0 mL·min^-1,柱温35℃,检测波长280 nm。结果:随炖制时间的增加,二苯乙烯苷含量逐渐降低,与8 h时相比,至64 h时含量降低76%;大黄素-8-O-β-D-葡萄糖苷和大黄素甲醚-8-O-葡萄糖苷2种蒽醌苷含量先上升后下降,至24 h时达最高值,炖制40 h时降至与8 h近似的水平,之后略有波动;大黄素、大黄素甲醚2种蒽醌苷元含量先上升后下降,炖制32 h时达最大值,之后缓慢降低并趋于稳定。结论:炖制时间对制何首乌中各类成分含量的影响显著,且变化趋势不尽相同,应规范制何首乌饮片的炮制时间;同时,仅以二苯乙烯苷及蒽醌类成分作为制何首乌的指标性成分依据不够充分,应考虑增加多糖类等质量控制指标。

Objective:To investigate the effects of black bean juice with different stewing times on the appearance character and the content changes of effective components of Polygoni Multiflori Radix Praeparata.Method:HPLC was employed with Agilent ZORBAX Extend-C18 column(4.6 mm×250 mm,5μm),a gradient mobile phase of methanol(A)-water(B)was eluted(0-30 min,5%-100%A;30-40 min,100%A),the flow rate was 1.0 mL·min^-1,the injection volume was 10μL and the column temperature was 35℃,detection wavelength was set at 280 nm.The contents of stilbene glycoside,emodin,emodin methyl ether,emodin-8-O-β-Dglucoside and emodin methyl ether-8-O-glucoside in samples prepared at different processing times were simultaneously determined by HPLC.Result:The content of stilbene glycoside decreased gradually with the increase of stewing time,compared with 8 h,its content decreased by 76%at 64 h.The contents of emodin-8-O-β-D-glucoside and emodin methyl ether-8-O-glucoside increased first,and then decreased,reaching the highest value at 24 h,and then decreased to the level similar to the content of 8 h after 40 h,and then fluctuated slightly.The contents of emodin and emodin methyl ether increased first,and then decreased,reached the maximum when stewed for 32 h,then decreased slowly and tended to be stable.Conclusion:The stewing time has significant influence on the content of various components in Polygoni Multiflori Radix Praeparata,and the changing trend is different,the processing time of Polygoni Multiflori Radix Praeparata shall be standardized.At the same time,it is not sufficient to take stilbene glycoside and anthraquinones as the indicator ingredients for this decoction pieces,the quality control indicators such as polysaccharides shall be considered.