Biological,antibacterial activities and electrochemical behavior of borided commercially pure titanium in BSA-containing PBS

含BSA的PBS中硼化工业纯钛的生物活性、抗菌活性和电化学行为

The effects of boride coating on the bioactivity, antibacterial activity, and electrochemical behavior of commercially pure titanium(CP-Ti) in phosphate buffer solution(PBS) with bovine serum albumin(BSA) were studied. The grazing incidence X-ray diffraction(GIXRD) pattern confirmed the formation of a Ti B/Ti B2 coating via boriding process. Scanning electron microscopy(SEM) observation indicated that the Ti B2 cross-linked particles covered the Ti B whiskers. Water contact angle measurements revealed that boriding led to the formation of a surface with intermediate water affinity. Potentiodynamic polarization(PDP) assays demonstrated that the Ti B/Ti B2 coating had acceptable passivation behavior in BSA-containing PBS. Electrochemical impedance spectroscopy(EIS) measurements revealed that the passivation behavior of the CP-Ti and the borided samples was improved by increasing exposure time. Based on the Mott-Schottky(M-S) tests, it was realized that the charge carriers of passive films of both samples decreased with increasing exposure time in BSA-containing PBS. The bioactivity test results in a simulated body fluid showed that the Ti B/Ti B2 coating switched the CP-Ti from bioinert to bioactive material. Finally, the antibacterial activity test of the Ti B/Ti B2 coating against Escherichia coli and Staphylococcus aureus indicated 99% antibacterial activity.

研究硼化物涂层对含牛血清白蛋白(BSA)的磷酸盐缓冲液(PBS)中工业纯钛(CP-Ti)的生物活性、抗菌活性和电化学行为的影响。掠入射X射线衍射(GIXRD)谱证实,经硼化处理已形成TiB/TiB2涂层。扫描电镜(SEM)测试表明,TiB2交联颗粒覆盖TiB晶须。水接触角测量发现,硼化处理导致形成具有中间亲水性的表面。动电位极化(PDP)测试表明,在含BSA的PBS中TiB/TiB2涂层具有可接受的钝化行为。电化学阻抗谱(EIS)测量表明,延长暴露时间使CP-Ti和硼化样品的钝化行为得到改善。根据Mott-Schottky(M-S)分析结果,两种样品钝化膜的载流子均随样品在含BSA的PBS中暴露时间的延长而减少。模拟体液中的生物活性测试表明,TiB/TiB2涂层使CP-Ti从生物惰性材料转变成生物活性材料。最后,对大肠杆菌和金黄色葡萄球菌的抗菌活性测试表明,TiB/TiB2涂层的抗菌活性为99%。