下调MCT1表达抑制宫颈癌Hela细胞增殖并促进其凋亡

Down regulation of MCT1 expression inhibits proliferation and promotes apoptosis of Hela cells

目的探讨下调单羧酸转运蛋白1(monocarboxylate transporter 1,MCT1)表达对宫颈癌Hela细胞增殖和凋亡的影响及其机制。方法将体外培养的Hela细胞分为未转染组、阴性对照组和MCT1-siRNA组,Western blot检测Hela细胞中MCT1、Bcl-2和Bax蛋白表达水平,MTT法检测Hela细胞增殖活力,流式细胞仪检测Hela细胞周期分布和凋亡率。结果与未转染组比较,阴性对照组Hela细胞中MCT1、Bcl-2、Bax蛋白表达水平和细胞增殖、周期分布以及细胞凋亡率差异均无统计学意义(P>0.05),MCT1-siRNA组细胞中MCT1和Bcl-2蛋白表达水平、细胞增殖活力和细胞在S期所占百分比均明显降低,Bax蛋白表达水平、细胞在G0/G1期所占百分比和细胞凋亡率均明显升高(P<0.05)。结论下调MCT1表达可通过诱导细胞周期阻滞抑制Hela细胞增殖,并通过上调Bax和下调Bcl-2蛋白表达促进Hela细胞凋亡。

Objective To investigate the effect of down-regulation of monocarboxylate transporter1(MCT1)on the proliferation and apoptosis of Hela cells and its mechanism.Methods HeLa cells were divided into three groups:untransfected group,negative control group and MCT1 siRNA group.The expression of MCT1,Bcl-2 and Bax protein in Hela cells was detected by Western blot,Hela cell proliferation was detected by MTT,Hela cell cycle distribution and apoptosis rate were detected by flow cytometry.Results Compared with the control group,the expression level of MCT1,Bcl-2,Bax protein,cell proliferation,cycle distribution and apoptosis rate of Hela cells in the negative control group had no significant difference(P>0.05),but the expression level of MCT1 and Bcl-2 protein,cell proliferation activity and the percentage of cells in S phase in the MCT1-siRNA group were significantly reduced,while the expression level of Bax protein and the percentage of cells in S phase were significantly reduced The percentage and apoptosis rate in G0/G1 phase were significantly increased(P<0.05).Conclusion Down regulation of MCT1 expression can inhibit Hela cell proliferation by inducing cell cycle arrest,and promote Hela cell apoptosis by up regulation of Bax and down regulation of Bcl-2 protein expression.