深圳市宝安区变应性鼻炎患者血清中维生素D表达及其受体基因rs7975232位点多态性分析

Expression of vitamin D and polymorphism analysis of vitamin D receptor gene rs7975232 in serum of allergic rhinitis patients at Baoan District in Shenzhen

目的了解深圳市宝安区变应性鼻炎(AR)患者血清中维生素D(VitD)表达情况,并探讨其受体(VDR)基因rs7975232位点多态性与AR之间的遗传易感性。方法选择2017年8月至2019年6月来深圳市宝安区福永人民医院就诊并确诊为AR患者286例(AR组),其中男性172例,女性114例;年龄7~69岁,平均年龄37.15岁。同时选择来医院体检的健康人群120例(对照组),其中男性74例,女性46例;年龄8~67岁,平均年龄36.07岁。采用化学发光免疫分析法检测血清中25-羟基维生素D3 [25-(OH)VitD3]水平,同时采用聚合酶链反应-限制性片段长度多态性(PCRRFLP)法检测VDR基因rs7975232位点多态性。结果 AR组患者血清中25-(OH)VitD3水平为(36.01±10.27) nmol/L,明显低于对照组[(61.72±23.05) nmol/L],差异有统计学意义(t=4.096 5,P <0.05);中-重度AR患者血清中25-(OH)-VitD3水平为(27.53±7.15) nmol/L,明显低于轻度AR患者[(41.92±12.84) nmol/L],差异有统计学意义(t=3.218 2,P <0.05)。AR组VDR基因rs7975232位点的AA基因型及A等位基因检出率分别为33.21%、54.55%,明显高于对照组(19.17%、37.92%),差异有统计学意义(χ^2=3.162 4、4.098 7,P <0.05);VDR基因rs7975232位点AA基因型的AR患者血清中25-(OH)VitD3水平为(21.95±8.36) nmol/L,明显低于AC和CC基因型[(32.91±11.52) nmol/L、(45.16±13.87) nmol/L],差异有统计学意义(t=3.816 2、6.052 4,P <0.05)。结论 AR患者血清中25-(OH)VitD3水平明显低于健康人群,随着AR病情加重而降低;AR患者VDR基因rs7975232位点的AA基因型及A等位基因检出率明显升高,且携带AA基因型的AR患者血清中25-(OH)VitD3水平明显降低,可能是深圳市宝安区AR发病的危险遗传易感基因之一。

Objective To investigate serum vitamin D(VitD) expression of allergic rhinitis(AR) patients at Baoan District in Shenzhen, and explore genetic susceptibility to AR associated with VitD receptor(VDR) rs7975232 polymorphism. Methods From August 2017 to June 2019, a total of 286 AR patients(AR group, which included 172 males and 114 females;aged 7-69 years old with mean age of 37.15 years old) were enrolled. In the same period, 120 healthy subjects underwent health examination were set as control group(included 74 males and 46 females;aged 8-67 years old with mean age of 36.07 years old).The chemiluminescence immunoassay was used to detect level of 25-(OH)VitD3 in serum, and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method was used to detect VDR gene rs7975232 loci polymorphism.Results The serum level of 25-(OH)VitD3 in AR group was(36.01 ± 10.27) nmol/L, which was significantly lower than that of control group[(61.72 ± 23.05) nmol/L], and the difference was statistically significant(t = 4.096 5, P < 0.05). The serum level of25-(OH)VitD3 in moderate-severe AR patients[(27.53 ± 7.15) nmol/L] was significantly lower than that of mild AR patients[(41.92 ± 12.84) nmol/L], and the difference was statistically significant(t = 3.218 2, P < 0.05). The AA genotype and A allele detection rate of rs7975232 of VDR gene in AR group were 33.21 % and 54.55 %, respectively, which were significantly higher than that in control group(19.17 % and 37.92 %), and the differences were statistically significant(χ^2= 3.162 4, 4.098 7, P < 0.05).The serum level of 25-(OH)VitD3 in AR patients with AA genotype at rs7975232 of VDR gene was(21.95 ± 8.36) nmol/L,which was significantly lower than that of AC and CC genotypes[(32.91 ± 11.52) nmol/L and(45.16 ± 13.87) nmol/L], and the difference was statistically significant(t = 3.816 2, 6.052 4, P < 0.05). Conclusion It is demonstrated that the serum 25-(OH)VitD3 level in AR patients is significantly lower than that in healthy people, and decrease with the aggravation of AR. The detection rate of AA genotype and A allele at rs7975232 of VDR gene in AR patients is significantly increased, and serum level of 25-(OH)VitD3 in AR patients with AA genotype is significantly reduced, which may be one of the risk genetic susceptibility gene for AR incidence at Baoan district in Shenzhen.