章鱼内脏鱼油的提取及品质分析

Extraction and quality analysis of octopus viscera fish oil

对章鱼内脏的基本营养成分、脂质组成及脂肪酸组成进行分析,比较不同提取方法对油脂提取率的影响,确定了酶解法与溶剂提取法结合的提取方法。采用单因素试验和正交试验优化酶解工艺条件,确定最优酶解工艺条件为采用中性蛋白酶进行酶解、酶解温度50℃、料液比1∶0.5、加酶量3500 U/g、酶解时间4 h,在此条件下油脂提取率为74.81%。通过脱胶、脱酸、脱胆固醇、脱色、脱臭得到精制章鱼内脏鱼油,精制章鱼内脏鱼油的理化指标基本达到SC/T 3502-2016精制鱼油二级标准,油脂脂肪酸组成未发生改变,鱼油中多不饱和脂肪酸含量为50.51%,EPA、DHA含量达37.30%。章鱼内脏鱼油具有较高的应用价值和开发前景。

The basic nutritional component,lipid composition and fatty acid composition of octopus viscera were analyzed,and the effects of different extraction methods on oil extraction rate were compared.The method of enzymatic hydrolysis combined with solvent extraction was adopted.In addition,the optimal conditions of enzymolysis were optimized by single factor experiment and orthogonal experiment.The optimal conditions of enzymolysis were obtained as follows:using neutral protease,enzymolysis temperature50℃,ratio of solid to liquid 1∶0.5,dosage of enzyme 3500 U/g,enzymolysis time 4 h.Under these conditions,the oil extraction rate was 74.81%.The refined octopus viscera fish oil was obtained by a series of refining process including degumming,deacidification,cholesterol removal,bleaching and deodorization.The physicochemical properties of the refined octopus viscera fish oil basically reached the second grade standard of refined fish oil(SC/T 3502-2016),and no obvious change was observed in fatty acid composition of the fish oil.The polyunsaturated fatty acid content of the refined fish oil was50.51%,and the total content of EPA and DHA was 37.30%.Octopus viscera fish oil had high application value and development prospect.