MTHFR、MTRR基因多态性与不明原因复发性流产研究

Correlation between MTHFR and MTRR Gene Polymorphisms and Unexplained Recurrent Abortion

目的探讨5,10-亚甲基四氢叶酸还原酶(MTHFR)基因C677T位点、A1298C位点以及甲硫氨酸合成酶还原酶(MTRR)基因A66G位点多态性与不明原因复发性流产关系。方法采用病例对照研究办法,方便选取2017年7月—2019年7月在该院生殖中心就诊的原因不明胎停育≥2次的130例患者为病例组,122例在该院产检孕周大于28周,无并发症的孕妇或者生育大于1个小孩的妇女且未发生过流产的患者作为对照组,比较病例组与对照组基因型,分析不明原因复发性流产与MTHFR基因C677T位点、A1298C位点和MTRR基因A66G位点多态性关系。结果MTHFR基因C677T位点中,病例组CC基因频率为30.77%,对照组CC基因频率为57.38%,差异有统计学意义(χ^2=18.115,P<0.05);病例组CT基因频率为55.38%,对照组CT基因频率为37.70%,差异有统计学意义(χ^2=7.900,P<0.05);病例组TT基因频率为13.85%,对照组TT基因频率为4.92%,差异有统计学意义(χ^2=5.821,P<0.05);病例组C等位基因频率为58.46%,对照组C等位基因频率为76.23%,差异有统计学意义(χ^2=32.389,P<0.05);病例组T等位基因频率为41.54%,对照组T等位基因频率为23.77%,差异有统计学意义(χ^2=32.389,P<0.05)。两组患者在CC、CT、TT3种基因型、C等位基因、T等位基因的分布差异有统计学意义(P<0.05)。MTHFR基因A1298C位点、MTRR基因A66G位点多态性病例组和对照组差异无统计学意义(P>0.05)。结论C677T的TT型是复发性流产的高危因素,MTHFR基因多态性与不明原因复发性流产有一定的关系,MTHFR基因A1298C位点、MTRR基因A66G位点多态性与不明原因复发性流产无明确关系。

Objective To discuss 5,10-Polymorphisms of C677T,A1298C,and A66G of the methionine synthase reductase(MTRR)gene in the methylenetetrahydrofolate reductase(MTHFR)gene were associated with unexplained recurrent abortion.Methods A case-case-control study was adopted.130 patients with unexplained fetal discontinuations of≥2 visits to the reproductive center of our hospital from July 2017 to July 2019 were selected as the case group,and 122 patients with gestational age greater than 28 weeks,uncomplicated pregnant women or women with more than one child who have not had an abortion were used as the control group.Genotypes were compared between the case group and the control group,and the polymorphism of MTHFR gene C677T,A1298C and MTRR gene A66G were analyzed.Results At the C677T locus of the MTHFR gene,the CC gene frequency in the case group was 30.77%,the CC gene frequency in the control group was 57.38%,the difference was statistically significant(χ^2=18.115,P<0.05);the CT gene frequency in the case group was 55.38%,and the CT gene frequency in the control group was 37.70%,the difference was statistically significant(χ^2=7.900,P<0.05);case group TT gene frequency was 13.85%,control group TT gene frequency was 4.92%,the difference was statistically significant(χ^2=5.821,P<0.05);case group C allele frequency was 58.46%,control group C of allele frequency was 76.23%,the difference was statistically significant(χ^2=32.389,P<0.05);the T allele frequency in the case group was 41.54%,and the T allele frequency in the control group was 23.77%,the difference was statistically significant(χ^2=32.389,P<0.05).The differences in the distribution of CC,CT,and TT genotypes,C alleles,and T alleles between the two groups of patients were statistically significant(P<0.05).There was no significant difference between MTHFR gene A1298C locus and MTRR gene A66G loci polymorphism case group and control group(P>0.05).Conclusion Unexplained recurrent miscarriage is related to the MTHFR gene C677T polymorphism,and has no clear relationship with the MTHFR gene A1298C locus and MTRR gene A66G polymorphism.