结肠腺癌组织中ARMCX1的表达及临床意义

Expression and clinical significance of ARMCX1 in colon adenocarcinoma

目的探讨结肠腺癌组织中ARM重复X连锁蛋白1(ARMCX1)的表达及临床意义。方法收集65例结肠腺癌患者癌组织(研究组)及其癌旁正常结肠黏膜组织(对照组),应用免疫组化染色和Western blot检测两组ARMCX1蛋白的表达,应用实时荧光定量PCR检测两组ARMCX1 mRNA的表达。分析结肠腺癌组织ARMCX1阳性表达与临床病理特征的相关性。随访5年,观察结肠癌患者生存情况。结果研究组ARMCX1阳性表达率高于对照组(53.8%vs.9.2%)(P<0.05)。研究组ARMCX1蛋白和mRNA表达高于对照组(1.15±0.21 vs.0.69±0.19和0.85±0.12 vs.0.72±0.14)(P<0.05)。发生脉管和神经累犯以及有淋巴结转移的结肠腺癌患者癌组织ARMCX1阳性表达率高于未发生者(P<0.05)。增殖指数≥25%的结肠腺癌患者癌组织ARMCX1阳性表达率高于增殖指数<25%者(P<0.05)。随访期间,存活20例,死亡42例,失访3例;死亡患者术后的生存时间为8~60(35.3±8.9)个月。与结肠腺癌组织ARMCX1阴性表达患者相比,阳性表达患者5年生存率低(P<0.05)。结论ARMCX1在结肠腺癌组织中表达增高,可能对病变形成和进展有促进作用。

Objective To investigate the expression and clinical significance of ARM repeat X-linked protein 1(ARMCX1)in colon adenocarcinoma.Methods The cancer tissues(group A)and normal colon mucosa tissues(group B)of 65 patients with colon adenocarcinoma were collected.The protein and mRNA expressions of ARMCX1 of two groups were detected by immunohistochemistry,Western blot and qRT-PCR,respectively.The correlation between the positive expression of ARMCX1 in colon adenocarcinoma and clinicopathological characteristics was analyzed.The patients were followed up for 5 years.Five-year survial rate in the patients with colon adenocarcinoma was observed.Results The positive rate of ARMCX1 in group A was higher than that in group B(53.8%vs.9.2%)(P<0.05).The protein and mRNA expressions of ARMCX1 in group A were higher than those in group B(1.15±0.21 vs.0.69±0.19 and 0.85±0.12 vs.0.72±0.14)(P<0.05).The positive expression rate of ARMCX1 in the patients with vascular and nerve invension and lymph node metastasis was higher than that in the patients without(P<0.05).The positive expression rate of ARMCX1 in the patients with proliferation index≥25%was higher than that in the patients with proliferation index<25%(P<0.05).During 5 years of follow-up,20 cases survived,42 cases died,and 3 cases were lost follow-up.The postoperative survival times of the dead patients were 8-60(35.3±8.9)months.Compared with patients with negative expression of ARMCX1 in colon adenocarcinoma,patients with ARMCX1 positive expression had lower five-year survial rate(P<0.05).Conclusion ARMCX1 is highly expressed in colon adenocarcinoma,which may promote the occurrence and progression of colorectal adenocarcinoma.