斑节对虾幼虾力竭运动后呼吸代谢变化研究

Study on respiratory metabolism changes of juvenile Penaeus monodon following strenuous activity

文章以斑节对虾(Penaeus monodon)为研究对象,在实验室水槽模拟幼虾力竭运动情况,探究幼虾力竭运动后的呼吸代谢恢复规律,以期为斑节对虾养殖生产和增殖放流提供科学依据。结果显示,在力竭后的各恢复时间点,斑节对虾幼虾肝胰腺总蛋白质(TP)、乳酸(LA),以及谷草转氨酶(AST)、谷丙转氨酶(ALT)、乳酸脱氢酶(LDH)和琥珀酸脱氢酶(SDH)活性均存在显著差异(P<0.05);腹部肌肉中LA、LDH活性存在显著差异。肝胰腺TP在恢复后第4小时显著高于对照组,第48小时恢复至对照组水平;LA在恢复后第12小时显著高于对照组,第24小时恢复至对照组水平;AST和ALT活性在运动后第8至第12小时内显著上升,第24小时恢复至对照组水平;LDH和SDH活性持续上升,恢复后第12小时显著高于对照组,第48小时恢复至对照组水平。肌肉糖原(GLY)在运动后显著下降,恢复后第12小时恢复至对照组水平;LA在运动后显著升高,第8小时下降至对照组水平;LDH活性运动后显著升高,第1至第12小时内持续升高,第72小时仍处于显著高于对照组水平。幼虾在力竭运动后无氧代谢显著增强,第8小时可再次进行力竭运动;肝胰腺为幼虾清除代谢物的器官,对运动后呼吸代谢恢复起到重要作用。

In order to provide scientific basis for the breeding and releasing of Penaeus monodon,we simulated the exhausted movement of the juveniles in the laboratory flume,then investigated their respiratory metabolic recovery after exhaustion exercise.With different recovery times after exhaustive exercise,there were significant changes in the total protein content(TP)and lactic acid(LA)concentrations as well as the activities of aspartate aminotransferase(AST),alanine aminotransferase(ALT),lactate dehydrogenase(LDH)and succinate dehydrogenase(SDH)in the hepatopancreas of the juveniles(P<0.05).There was significant difference in the LA concentration and LDH activity in abdominal muscles.The TP concentration in hepatopancreas was significantly higher than that of the control group after 4 h of exhaustive exercise,and returned to the control level at 48th hour.The LA concentration of hepatopancreas was significantly higher than that of the control group at 12th hour,and returned to the control level at 24th hour.The activities of AST and ALT in hepatopancreas increased significantly during the 8th to 12th hour,and returned to the control level at 24th hour.The activities of LDH and SDH increased and were significantly higher than that of the control group at 12th hour,and returned to the control level at 48th hour.The glycogen concentration(GLY)in the muscle decreased significantly after exhaustive exercise and returned to the control level at the 12th hour.The LA concentration increased significantly after exhaustive exercise and decreased to the control level at 8th hour.The LDH activity increased significantly after exhaustive exercise,and continued to increase during 1st to 12th hour,and remained significantly higher than that of the control group at 72th hour.The anaerobic metabolism of the juveniles was significantly enhanced after exhaustive exercise,and exhaustive exercise could be performed again after 8 h;heaptopancreas was the place where the juveniles cleared metabolites,and began to deal with internal environment imbalance after exercise.