斯钙素2在糖尿病视网膜病变中的表达和意义

Expression and significance of stanniocalcin 2 in diabetic retinopathy

目的:研究斯钙素2(STC2)在糖尿病视网膜病变(DR)大鼠玻璃体组织中的表达水平,并探究其与血管内皮生长因子(VEGF)的关系。方法:Wistar大鼠随机分为正常对照(control)组和DR组,DR模型通过注射链脲佐菌素构建而成。RT-qPCR、Western blot和ELISA检测大鼠玻璃体中STC2和VEGF的表达水平;用VEGFA处理大鼠视网膜神经节细胞,RT-qPCR、Western blot和ELISA检测STC2的表达水平;用STC2蛋白处理大鼠视网膜神经节细胞,RT-qPCR、Western blot和ELISA检测VEGF的表达水平,免疫共沉淀检测VEGF与STC2的相互作用。结果:相对于control组,DR模型大鼠玻璃体中STC2的mRNA和蛋白水平显著升高,VEGF的表达水平也显著升高(P<0.01),两者呈正相关;VEGFA可以诱导大鼠视网膜神经节细胞中STC2的表达,并促进其分泌;STC2蛋白可以诱导大鼠视网膜神经节细胞中VEGF的表达和分泌,并且VEGF与STC2存在蛋白相互作用。结论:STC2在DR大鼠玻璃体中表达显著升高,并且其表达与VEGF紧密相关。

AIM:To investigate the expression level of stanniocalcin 2(STC2)in vitreous tissues of rats with diabetic retinopathy(DR),and to explore the relationship between STC2 and vascular endothelial growth factor(VEGF).METHODS:Wistar rats were randomly divided into control group and DR group. The DR model was constructed by injection of streptozotocin. RT-qPCR,Western blot and ELISA were used to detect the expression levels of STC2 and VEGF in rat vitreous tissues. Rat retinal ganglion cells were treated with VEGFA,and the expression of STC2 was detected by RT-qPCR,Western blot and ELISA. The retinal ganglion cells were also treated with STC2 protein,and then the expression of VEGF was detected by RT-qPCR,Western blot and ELISA. Co-immunoprecipitation was used to detect the interaction between VEGF and STC2.RESULTS:Compared with control group,the mRNA and protein levels of STC2 were significantly increased in vitreous tissues of the rats with DR,and the expression level of VEGF was also significantly increased in DR group(P<0. 01). The expression level of STC2 was positively correlated with VEGF expression. VEGF induced the expression of STC2 in rat retinal ganglion cells and promoted its secretion. STC2 protein induced the expression and secretion of VEGF in rat retinal ganglion cells,and VEGF had certain interaction with STC2.CONCLUSION:STC2 expression is significantly increased in vitreous tissues of the rats with DR,and is closely related to VEGF.