Study on chemical composition of Viscum coloratum and its HPLC fingerprint in different harvest periods

The purpose of this study was to evaluate the similarity of chemical profile of Viscum loloratum harvested in different months using high performance liquid chromatography(HPLC)fingerprint and multivariate statistical analysis,and to determine the optimal harvest time of Viscum coloratum.8 compounds were isolated from Viscum coloratum by a variety of isolation techniques,3 of which were isolated from the genus Viscum L.for the first time.The fingerprint of Viscum coloratum was established by HPLC,and 16 common peaks were obtained,4 of which were identified by comparison with the reference substances.Results showed that the Viscum coloratum harvested in 6 months could be roughly divided into three groups based on the chemical profiles,and the Viscum coloratum harvested in January had higher levels of the marked compounds.The HPLC fingerprint objectively reveals the characteristics of Viscum coloratum harvested in different months,which can be used to evaluate and control the quality of Viscum coloratum harvested in different months.