摘要
选用N,N-碳酰二咪唑(CDI)方法活化聚乙二醇(PEG)的羟基,制备PEG修饰的聚乙烯亚胺(PEG-PEI),不仅具有较高的产率,还能降低实验成本和简化操作步骤,使基因传输载体综合实验在本科生阶段开展具有可行性。基因传输载体综合实验设计也纳入了载体聚合物的制备与表征、纳米粒子组装与表征、细胞毒性评价、载体负载和传输核酸能力的评价,使学生能较为系统地了解和掌握基因传输载体材料的设计、制备、组装和评价等方面的实验方法和技能。
To improve successful probability and simplify operation procedure,the CDI,an easily available reagent for carboxyl group activation,is used to activate hydroxyl-terminated polyethyleneglycol( PEG-OH). The PEG-grafted PEI polymer is prepared via the formation of amido linkage between PEG-CDI and PEI under mild condition,it allows to be applied as an integrated biomaterials experiment for students. Nanocomplex complexing pDNA is fabricated and characterized using several approaches including dynamic light scattering( DSL),cytotoxicity,and gene expression analyses. The integrated experiment is favor of training the student’s research ability and learning the knowledge and skills about the design,fabrication,and functional evaluation of biomaterials.
作者
程度
李晓霞
乔立红
CHENG Du;LI Xiaoxia;QIAO Lihong(School of Materials Science and Engineering,Sun Yatsen University,Guangzhou 510275,China)
出处
《实验室研究与探索》
CAS
北大核心
2020年第8期22-26,55,共6页
Research and Exploration In Laboratory
基金
国家自然科学基金面上项目(21875289)
科技部国家重点研发计划国际合作交流重点项目(2016YFE0117100)。
关键词
基因传输载体
CDI活化
功能评价
生物材料
gene delivery carrier
CDI activation
functional evaluation
biomaterials
