转录因子FoxO1与miR-142-3p在口腔鳞癌中的表达关系及意义

Expression relationship and significance of FoxO1 and miR-142-3p in oral squamous cell carcinoma

目的探讨转录因子叉头框转录因子O亚族1(FoxO1)与微小RNA-142-3p(miR-142-3p)在口腔鳞癌(OSCC)中的表达关系及意义。方法选取2016年5月~2018年6月于本院手术中留取的OSCC组织72份为观察组,选取50份形态学正常的癌旁口腔黏膜组织为对照组,比较两组FoxO1、miR-142-3p表达与OSCC临床病理特征的关系及二者的相关性,并采用COX回归模型分析OSCC患者预后不良事件的影响因素。结果观察组较对照组FoxO1阳性表达率、miR-142-3p表达均较低(P<0.05);高分化、中分化、低分化相比,FoxO1阳性表达率、miR-142-3p表达均依次降低(P<0.05);与Ⅰ~Ⅱ期相比,Ⅲ~Ⅳ期FoxO1阳性表达率、miR-142-3p表达均较低(P<0.05);与无淋巴结转移相比,淋巴结转移者FoxO1阳性表达率、miR-142-3p表达均较低(P<0.05)。Spearman结果显示,观察组FoxO1、miR-142-3p表达呈正相关(P<0.05)。单因素分析显示,性别、年龄、BMI、病理分级均与OSCC患者不良预后无关(P>0.05),临床分期、淋巴结转移、FoxO1、miR-142-3p均是OSCC患者不良预后的影响因素(P<0.05)。多因素分析显示,临床分期、淋巴结转移、FoxO1、miR-142-3p均是影响OSCC患者预后发生不良事件的独立危险因素(P<0.05)。结论FoxO1、miR-142-3p与OSCC的发生、发展及预后密切相关,FoxO1可能受miR-142-3p的靶向调控,这一结果可为病情评估及治疗靶点提供临床参考依据。

Objective To investigate the expression relationship between forkhead transcription factors of the O class 1(FoxO1)and microRNA-142-3p(miR-142-3p)in oral squamous cell carcinoma(OSCC)and their significance.Methods From May 2016 to June 2018,72 samples of OSCC tissue taken during the operation were selected as the observation group,and 50 normal morphologically adjacent oral mucosa tissues were selected as the control group.The relationships between the expressions of FoxO1,miR-142-3p and the clinicopathological characteristics of OSCC,and the correlation between them were compared.The influencing factors of prognosis adverse events of OSCC patients were analyzed by COX regression model.Results The positive expression rate of FoxO1 and miR-142-3p in the observation group were lower than those in the control group(P<0.05).Comparison between high differentiation,medium differentiation and low differentiation,the positive expression rate of FoxO1 and expression of miR-142-3p decreased in turn(P<0.05).Compared with stageⅠ~ⅡI,the positive expression rate of FoxO1 and expression of miR-142-3p in stageⅢ~Ⅳwere lower(P<0.05).Compared with non lymph node metastasis,the positive expression of FoxO1 and expression of miR-142-3p in lymph node metastasis were lower(P<0.05).Spearman showed that the expressions of FoxO1 and miR-142-3p in the observation group were positively correlated(P<0.05).Univariate analysis showed that gender,age,BMI and pathological grade were not related to the poor prognosis of OSCC(P>0.05),and the clinical stage,lymph node metastasis,FoxO1 and miR-142-3p were all the influencing factors of the poor prognosis of OSCC(P<0.05).Multivariate analysis showed that clinical stage,lymph node metastasis,FoxO1,miR-142-3p were all independent risk factors for adverse events in the prognosis of OSCC patients(P<0.05).Conclusion FoxO1 and miR-142-3p are closely related to the occurrence,development and prognosis of OSCC,FoxO1 may be targeted by miR-142-3p,and provide clinical reference for disease assessment and treatment target.