恢复时间对力竭训练后的斑节对虾幼虾的抗氧化功能的影响

Effect of Recovery Time on Antioxidant Capacity of Juvenile Penaeus monodon After Exhaustive Exercise

力竭运动常出现于虾类养殖过程和野生环境中,斑节对虾(Penaeus monodon)是中国沿海重要养殖和增殖放流品种,但尚无关于斑节对虾力竭运动后生理恢复过程报道。该研究以斑节对虾幼虾为实验对象,在室内水槽使用抄网追逐斑节对虾幼虾,模拟迫使其进行力竭运动实验,探究其力竭运动后抗氧化能力恢复过程。结果显示,力竭运动后0h,幼虾体内的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)和谷胱甘肽过氧化物酶(GSH-Px)的活性,总抗氧化能力(T-AOC)和丙二醛(MDA)含量,均与对照组无显著差异;1~12 h,SOD、CAT、POD活性、MDA含量和T-AOC持续下降,GSH-Px活性无显著变化;24 h,SOD、CAT和POD活性显著降低,GSH-Px活性、MDA含量和T-AOC显著升高;72 h,SOD和CAT活性仍显著低于对照组,GSH-Px、POD活性和MDA含量恢复至对照组,T-AOC显著高于对照组。在力竭运动过程中,幼虾的酶促抗氧化系统未被激活;幼虾的抗氧化能力在力竭运动后的24h内较低,不宜再次受刺激;幼虾的抗氧化能力在力竭后24 h后显著上升,并保持较长时间。

Exhaustive exercise often occurs in shrimp farming and in the wild.Giant tiger prawn(Penaeus monodon)is an important species in aquaculture and proliferation in China’s coastal areas.The study used dip nets to chase juvenile P.monodon in the indoor sink,simulated the exhaustive exercise experiment,and explored the recovery process of antioxidant capacity after exhaustive exercise;however,there was no report on the physiological recovery process after the exhaustive exercise of P.monodon.The results showed the activities of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD)and glutathione peroxidase(GSH-Px),total antioxidant capacity(T-AOC),and malondialdehyde(MDA)content in juvenile P.monodon at 0 h after exhaustive exercise.No significant difference from the control group was observed.At 1~12 h,the activities of SOD,CAT,POD,MDA content,and T-AOC continued to decrease,whereas the activity of GSH-Px did not change significantly.At 24 h,the activities of SOD,CAT,and POD were significantly lower than those of the control group,and the activity of GSH-Px,MDA content,and T-AOC were significantly higher than those of the control group.At 72 h,the activities of SOD and CAT were still significantly lower than those in the control group.The activities of GSH-Px,POD,and MDA content were restored in the control group,and T-AOC activity was still significantly higher compared to that in the control group.This indicated that the enzymatic antioxidant system of P.monodon was not activated during exhaustive exercise.At 24 h after exhaustive exercise,the antioxidant capacity at 72 h was still at a high level.It was observed that the enzymatic antioxidant system was not activated in juvenile P.monodon during exhaustive exercise.The antioxidant capacity of juvenile P.monodon is lower within 24 h after exhaustive exercise,then increases significantly after 24 h of exhaustion and remained at that level for a long time.Within 24 h after exercise,further stimulation of juvenile P.monodon should be avoided.