农田环境中农药残留比例型荧光传感系统研究

Proportional Fluorescence Sensing Analysis of Pesticide Residues in Agricultural Environment

为监测农业环境中有机磷农药的残留,从种养源头管控农产品安全,基于锆离子和1,2,4,5-四(4-羧苯基)苯(H4TCPB)合成了蓝色荧光金属有机框架材料(MOFs)ZrTCPB,并与红色荧光量子点QDs组装成双荧光QDs@MOFs复合物,基于ZrTCPB对有机磷农药特异性荧光淬灭效应,构建比例型荧光化学传感器系统,实现了有机磷农药的快速、灵敏、可视化检测。甲基对硫磷与对硫磷的检测限(LOD)分别为1.9μg/L和4.9μg/L,线性检测范围为0.005~2 mg/L。研究表明,该荧光分析法能有效用于农业环境水样中甲基对硫磷及对硫磷的现场快速测定,甲基对硫磷回收率为93.23%~116.46%,平均相对标准偏差(RSD)为5.29%,对硫磷回收率为92.52%~107.83%,平均RSD为5.74%。该方法在环境样品农药残留快速监测方面具有巨大的应用价值。

Monitoring organophosphorus pesticide residues in agricultural environment is of great significance for the safety control of agricultural products from the source of breeding and planting.Based on zirconium ions and 1,2,4,5-tetramine(4-carboxyphenyl)benzene(H4TCPB),a kind of blue fluorescent ZrTCPB metalorganic frameworks(MOFs)materials was synthesized.And the ZrTCPB MOFs and quantum dots(QDs,emission at 625 nm)was employed to develop a proportional fluorescent sensor using the QDs@MOFs complex.Based on the specific fluorescence quenching response of ZrTCPB MOFs to some kinds of organophosphorus pesticides,a proportional fluorescence chemical sensing technology was constructed to realize the rapid,sensitive and visual detection of organophosphorus pesticides such as methyl-parathion and parathion in agricultural environment.The limits of detection(LOD)for methyl-parathion and parathion were 1.9μg/L and 4.9μg/L,respectively,and the linear detection range was 0.005~2 mg/L.Based on the sensitive fluorescence quenching effect,it had a good specific detection ability for methyl-parathion and parathion.In addition,the fluorescence analysis method can be effectively applied to the rapid field determination of methyl-parathion and parathion in water samples of agricultural environment.The recovery rate of methyl-parathion was 93.23%~116.46%,with an average RSD of 5.29%,and the recovery rate of parathion was 92.52%~107.83%,with an average RSD of 5.74%.The results showed that the developed method had great application value in the rapid monitoring of pesticides residue in agricultural environmental samples.