三阴性乳腺癌组织亲嗜性病毒整合位点1和核转运蛋白基因2的表达及与临床病理特征和预后的关系

Relationship of EVI1 and KPNA2 expression with clinicopathological features and prognosis in triple negative breast cancer

目的探讨亲嗜性病毒整合位点1(EVI1)、核转运蛋白基因2(KPNA2)在三阴性乳腺癌(TNBC)的表达,分析EVI1、KPNA2表达与临床病理特征及预后的关系。方法选取2012年1月—2015年1月中国人民解放军联勤保障部队第983医院收集的73例TNBC患者手术切除的癌组织、癌旁组织及70例正常乳腺组织(对照组)的石蜡标本,采用免疫组织化学法检测EVI1、KPNA2的阳性表达。收集相关临床病理资料,分析EVI1、KPNA2表达与TNBC患者临床病理特征的关系,采用Kaplan-Meier生存曲线分析不同EVI1、KPNA2表达下TNBC患者无进展生存时间(PFS)及总生存时间(OS)差异。结果癌组织、癌旁组织和对照组EVI1、KPNA2阳性表达率比较,差异有统计学意义(P<0.05),TNBC癌组织中EVI1、KPNA2阳性表达率高于癌旁组织和对照组(P<0.05),癌旁组织和对照组EVI-1、KPNA2阳性表达率比较,差异无统计学意义(P>0.05)。不同组织学分级、淋巴结、Ki-67表达、脉管内癌栓TNBC患者的EVI1阳性表达率比较,差异有统计学意义(P<0.05),淋巴结是否转移的TNBC患者的KPNA2阳性表达率比较,差异有统计学意义(P<0.05)。Kaplan-Meier生存分析结果显示EVI1阳性表达、KPNA2阳性表达患者PFS、OS生存率均低于EVI1阴性表达、KPNA2阴性表达患者(P<0.05)。结论EVI1、KPNA2阳性表达与TNBC患者肿瘤恶性侵袭行为和预后不良有关,评价EVI1、KPNA2表达可为TNBC患者预后预测提供一定的依据。

Objective To investigate the expression of ecotropic virus integration site 1(EVI1)and karyopherin-α2(KPNA2)in three negative breast cancer(TNBC),and their relationship with the clinicopathological characteristics and prognosis.Methods Seventy three paraffin samples of cancer tissues and paracancerous tissues from TNBC patients and 70 normal breast tissue(control group)from January 2012 to January 2015 were collected.The expression of EVI1 and KPNA2 were detected by immunohistochemistry.The relevant clinicopathological data were collected,and the correlation between the expression of EVI1,KPNA2 and the clinicopathological parameters of TNBC were analyzed.Kaplan-Meier survival analysis was used to analyze the difference of progression free survival(PFS)and overall survival(OS)of TNBC patients under different expression of EVI1 and KPNA2.Results The positive expression rates of EVI1 and KPNA2 in the cancer tissues,adjacent tissues and the control group were statistically significant(P<0.05).The positive expression rates of EVI1 and KPNA2 in TNBC cancer tissues were higher than those in the adjacent tissues and the control group(P<0.05),while the difference in the positive expression rates of EVI-1 and KPNA2 in the adjacent tissues and the control group was not statistically significant(P>0.05).The positive expressions of EVI1 of TNBC patients with the different histological grade,lymph nodes and tumor thrombus were significantly different(P<0.05),and the expression of KPNA2 between TNBC patients with lymph nodes metastasis or not were significantly different(P<0.05).The results of Kaplan-Meier survival analysis showed that the survival rate of PFS and OS in patients with positive expression of EVI1 and KPNA2 were lower than those in patients with negative expression(P<0.05).Conclusion The positive expression of EVI1 and KPNA2 are related to the malignant invasion and poor prognosis of TNBC patients.Valuating the expression status of EVI1 and KPNA2 can provide a certain basis for the prognosis prediction of TNBC patients.