多囊卵巢综合征患者血清镍纹样蛋白水平检测的临床意义及与实验室指标的相关性研究

Clinical Significance of Serum Metrnl Protein Level Detection in Patients with Polycystic Ovary Syndrome and Its Correlation with Laboratory Indexes

目的探究多囊卵巢综合征(polycystic ovary syndrome,PCOS)患者血清镍纹样蛋白(meteorin-like protein,Metrnl)水平检测的临床意义及与实验室指标的相关性。方法选取2018年4月~2019年9月西安高新医院妇科收治的61例PCOS患者为观察组,另选取同期58例健康体检女性作为对照组。应用全自动生化分析仪检测两组的总胆固醇(total cholesterol,TC)、三酰甘油(triglyceride,TG)、空腹血糖(fasting blood glucose,FBG)、超敏C反应蛋白(hypersensitive C-reactive protein,hs-CRP)、游离睾酮(free testosterone,FT)、卵泡刺激素(follicle-stimulating hormone,FSH)和促黄体生成素(luteinizing hormone,LH)等水平。采用酶联免疫吸附法(ELISA)检测血清中Metrnl的水平。相关性分析采用Spearson相关性检验。比较两组的实验室指标,正常和肥胖人群中血清Metrnl水平,分析Metrnl表达水平与实验室指标的相关性。结果两组的FBG,TC,低密度脂蛋白胆固醇(low density lipoprotein-cholesterin,LDL-C)和高密度脂蛋白胆固醇(high density lipoprotein-cholesterol,HDL-C)等实验室指标差异均无统计学意义(t=0.12,1.95,0.87,0.48,均P>0.05),观察组的hs-CRP和FT水平高于对照组,观察组的Metrnl(98.4±32.2 pg/ml)显著高于对照组(65.3±27.7 pg/ml),差异具有统计学意义(t=5.36,13.20,5.91,均P<0.05)。对照组中正常(18.5 kg/m^(2)≤BMI<24 kg/m^(2))和肥胖(BMI≥24 kg/m^(2))人群血清Metrnl水平差异无统计学意义(t=3.70,P>0.05)。观察组中肥胖人群血清Metrnl水平显著高于正常人群,差异具有统计学意义(t=11.41,P<0.05)。Metrnl表达水平与hs-CRP,FT和BMI呈正相关关系(r=0.507,0.309和0.391,均P=0.000)。结论Metrnl可作为PCOS潜在的生物学标记物,其表达水平与患者的hs-CRP,FT和BMI呈正相关。

Objective To explore the clinical significance of the detection of serum me teorin-like protein(Metrnl)level in patients with polycystic ovary syndrome(PCOS)and its correlation with laboratory indicators.Methods A total of 61 PCOS patients admitted to the Department of Gynaecology of Xi’an Gaoxin Hospital from April 2018 to September 2019 were selected as the observation group,and 58 healthy women in the same period were selected as the control group.Appling automatic biochemical analyzer test two groups of total cholesterol(TC),triglyceride(TG),fasting blood glucose(FBG),hypersensitive C-reactive protein(hs-CRP),free testosterone(FT),follicle-stimulating hormone(FSH)and luteinizing hormone(LH).Using ELISA to detect the levels of serum Metrnl.Spearson correlation test was used for correlation analysis.The laboratory indicators,the serum Metrnl levels in normal an d obese people were compared between the two groups,and the correlation between Metrnl expression levels and relevant laboratory indicators was analyzed.Results There was no significant difference in FBG,TC,low density lipoprotein-cholesterin(LDL-C)and high density lipoprotein-cholesterol(HDL-C)between the two groups(t=0.12,1.95,0.87,0.48,all P>0.05).The hs-CRP and FT levels in the observation group were higher than those in the control group,the Metrnl 98.4±32.2 pg/ml in the observation group was significantly higher than that in the control group(65.3±27.7 pg/ml),and the difference were statistically significant(t=5.36,13.20,5.91,all P<0.05).In the control group,there was no significant difference in serum Metrnl level between normal(18.5 kg/m^(2)≤BMI<24 kg/m^(2))and obese(BMI≥24 kg/m^(2))groups(t=3.70,P>0.05).In the observation group,the serum Metrnl level of obese people was significantly higher than that of normal people,and the difference was statistically significant(t=11.41,P<0.05).Metrnl expression level was positively correlated with hs-CRP,FT and BMI(r=0.507,0.309 and 0.391,all P=0.000).Conclusion Metrnl can be used as a potential biological marker of PCOS,and its expression level is positively correlated with pa tients hs-CRP,FT and BMI.