芹菜茎基腐病病原菌鉴定、消长动态调查与防治药剂筛选

Identification of causal agent and dynamics survey of celery stalk basal rot and screening of fungicides

为探明芹菜茎基腐病科学防控技术,于2017—2018年在浙江省杭州市系统观测了芹菜茎基腐病田间病情自然消长动态;采取组织分离、形态和系统发育分析、菌丝悬浮液接种等方法对病原菌进行了分离鉴定和致病性测定;采用菌丝生长速率法测定了29种常用杀菌剂对芹菜茎基腐病病原菌的抑制率,并开展了相关田间药效试验。结果表明,芹菜茎基腐病在芹菜上普遍发生,6—9月是该病的发病高峰期;引起芹菜茎基腐病的病原菌为尖孢镰刀菌,甲基硫菌灵和戊唑醇及其复配制剂对芹菜茎基腐病菌的菌丝生长抑制效果最佳,75%肟菌·戊唑醇WDG或70%甲基硫菌灵WP对芹菜茎基腐病的田间防效较好。

In order to characterize the causal agent and occurrence of celery stalk basal rot in Hangzhou,Zhejiang province and effective fungicides,the dynamic survey of celery stalk rot in field was investigated in 2017-2018,the pathogen was isolated and identified by tissue separation,morphological and phylogenetic analysis and its pathogenicity was determined by mycelial suspension inoculation,the inhibition of 29 common fungicides were determined by mycelial growth rate method,and field control experiment were conducted as well.The results showed that frequent occurrence of celery stalk basal rot was during June to September.Fusarium oxysporum was the pathogenic fungus that caused celery stalk basal rot.In vitro screening test showed that thiophanate-methyl and tebuconazoles fungicides had the best inhibitory effect on mycelial growth of pathogenic F.oxysporum,and field application of 75%trifloxystrobin·tebuconazole WDG and 70%thiophanate-methyl WP had better control effect on celery stalk basal rot.