雷洛昔芬对骨质疏松性颌骨骨折大鼠骨折愈合及OPG/RANKL/RANK系统的影响

Effects of raloxifene on fracture healing and OPG/RANKL/RANK system in rats with osteoporotic jaw fracture

目的:分析雷洛昔芬对骨质疏松性颌骨骨折大鼠骨折愈合及骨保护蛋白(OPG)/核因子κB的受体活化因子配体(RANKL)/核因子κB的受体活化因子(RANK)系统的影响。方法:采用SPF级SD大鼠建立骨质疏松性颌骨骨折模型。建模成功后,以雷洛昔芬12 mg/kg灌胃干预4周。X线摄片检查骨折愈合情况,双能X线骨密度测量仪测量骨痂区骨密度(BMD),H-E染色检查骨痂区骨组织形态,Western免疫印迹检测OPG、RANKL、RANK蛋白表达情况。采用SPSS 22.0软件包对数据进行统计学分析。结果:雷洛昔芬组术后2周、4周骨折线恢复较模型组优,H-E染色显示骨痂区骨组织骨小梁、骨基质样团块较模型组多,术后4周与空白对照组相当。雷洛昔芬组术后2、4周骨痂区BMD及OPG蛋白相对表达量显著高于模型组,RANKL、RANK蛋白相对表达量显著低于模型组(P<0.05),术后4周与空白对照组相当(P>0.05)。结论:雷洛昔芬能调节OPG/RANKL/RANK系统,促进骨质疏松性颌骨骨折大鼠的骨折愈合。

PURPOSE: To investigate the effects of raloxifene on fracture healing and osteoprotegerin(OPG)/receptor activator of NF-κB ligand(RANKL)/receptor activator of NF-κB(RANK) system in rats with osteoporotic jaw fracture.METHODS: SPF SD rats were used to establish an osteoporotic jaw fracture model. After successful establishment of the model, 12 mg/kg raloxifene were administered intragastrically for 4 weeks. X-ray film was taken to check the fracture healing. Bone mineral density(BMD) was measured by dual energy X-ray absorptiometry. H-E staining was used to exam-ine the morphology of callus. Western blot was used to detect the expression of OPG, RANKL and RANK. SPSS 22.0 software package was used for data analysis. RESULTS: The fracture line recovery of the raloxifene group was better than that of the model group 2 and 4 weeks after surgery. H-E staining showed more bone trabecula and bone matrix-like masses in the callus area than the model group, which was similar to that in blank control group 4 weeks after operation.The BMD and relative expression of OPG protein in raloxifene group were significantly higher than those in model group at2 and 4 weeks after operation, and the relative expression of RANKL and RANK protein were significantly lower than those in model group(P <0.05), which were similar to those in blank control group 4 weeks after operation(P >0.05).CONCLUSIONS: Raloxifene can regulate OPG/RANKL/RANK system and promote fracture healing in rats with osteoporotic jaw fracture.