丹参当归配伍对丹参水溶性和脂溶性成分提取的影响及其集成工艺研究

Study on Extraction and Purification Technology of Active Components of Danshen(Salviae Miltiorrhizae Radix Et Rhizoma) and Danggui(Angelicae Sinensis Radix) after Compatibility

目的通过正交和数学建模思想优化开展丹参与当归配伍对丹参水溶性和脂溶性成分提取的影响及其集成工艺研究,并探讨其配伍意义。方法利用正交设计法,分别以丹酚酸B和丹参酮ⅡA为指标,考察料液比、提取时间、提取温度及丹参当归不同比例配伍对丹参中水溶性和脂溶性成分提取的影响,并结合数学分析,优化同时提取两种成分的集成工艺;并观察丹参当归配伍前后对丹参有效成分的影响。结果丹参当归配伍后其集成最佳工艺为:料液比为1∶30,提取2 h,提取温度70℃,配伍比例1∶2(丹参∶当归);配伍后丹参中丹酚酸B和丹参酮ⅡA的提取率比配伍前分别提高69.78%、54.20%。结论集成最佳工艺可为含有丹参当归的复方制剂开发研究及工业化生产提供参考,也为中医药配伍从成分含量变化规律提供了新的研究思路,有助于指导临床合理配伍用药。

Objective To optimize the effects of compatibility of Danshen(Salviae Miltiorrhizae Radix Et Rhizoma) and Danggui(Angelicae Sinensis Radix) on the extraction of water-soluble and fat soluble components of Danshen(Salviae Miltiorrhizae Radix Et Rhizoma) and the integrated process by orthogonal design and mathematical modeling and discuss the significance of compatibility. Methods With salvianolic acid B and tanshinone ⅡA as indexes, the effects of solid-liquid ratio, extraction time, extraction temperature and different proportions of Danshen(Salviae Miltiorrhizae Radix Et Rhizoma) and Danggui(Angelicae Sinensis Radix) on the extraction of water-soluble and fat soluble components in Danshen(Salviae Miltiorrhizae Radix Et Rhizoma) were investigated by orthogonal design. Combined with mathematical analysis, the integrated process of simultaneous extraction of the two components was optimized, and the effects of Danshen(Salviae Miltiorrhizae Radix Et Rhizoma) and Danggui(Angelicae Sinensis Radix) on the effective components of Danshen(Salviae Miltiorrhizae Radix Et Rhizoma) were observed. Results The optimal integration conditions after compatibility were as follows. The solid-liquid ratio was 1∶30,extracting for 2 h. The compatibility ratio was 1∶2[Danshen(Salviae Miltiorrhizae Radix Et Rhizoma):Danggui(Angelicae Sinensis Radix)] and the extracting temperature was 70 ℃. After the compatibility, the extraction yield of salvianolic acid B and tanshinone ⅡA increased by 69.78% and 54.20%,respectively. Conclusion The optimal integration conditions in this paper provided reference for development and industrial production of compound preparation containing Danshen(Salviae Miltiorrhizae Radix Et Rhizoma) and Danggui(Angelicae Sinensis Radix). Moreover, the model of extraction conditions and extraction rate established in this paper is in line with the reality. The best extraction process conditions can be obtained through the model, which is conducive to the optimal extraction of these two substances. Under the optimal extraction conditions, the extraction rate is higher.